LOCUS       SIU01092.1               172 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            RESUSCITATION-PROMOTING FACTOR RPFE protein.
ACCESSION   LT708304-2506
PROTEIN_ID  SIU01092.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="rpfE"
                     /locus_tag="BQ2027_MB2477C"
                     /note="Mb2477c, rpfE, len: 172 aa. Equivalent to Rv2450c,
                     len: 172 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.4% identity in 172 aa overlap). Probable rpfE,
                     resuscitation-promoting factor (see first citation below),
                     similar to O86308|Z96935|MLRPF_1 RPF PROTEIN PRECURSOR
                     from Micrococcus luteus (220 aa), FASTA scores: opt: 291,
                     E(): 3e-7, (48.75% identity in 80 aa overlap). C-terminus
                     is similar to other Mycobacterial rpf proteins e.g.
                     O05594|Rv1009|MTCI237.26|RPFB PROBABLE
                     RESUSCITATION-PROMOTING FACTOR from Mycobacterium
                     tuberculosis (362 aa), FASTA scores: opt: 344, E():
                     1.4e-09, (42.85% identity in 147 aa overlap); etc.
                     C-terminal region similar to N-terminal region of
                     Q9F2Q2|SCE41.06c PUTATIVE SECRETED PROTEIN from
                     Streptomyces coelicolor (244 aa), FASTA scores: opt: 355,
                     E(): 3.1e-10, (56.65% identity in 90 aa overlap). Also
                     similar to Q9F2Q1|SCE41.07c PUTATIVE SECRETED PROTEIN from
                     Streptomyces coelicolor (near Q9F2Q2|SCE41.06c) (341 aa)
                     FASTA scores: opt: 317, E(): 2.5e-08, (51.7% identity in
                     87 aa overlap). With Mycobacterium leprae, high similarity
                     between the two corresponding C-terminal regions of two
                     HYPOTHETICAL PROTEINS, Q9CD53|ML0240 (375 aa), FASTA
                     scores: opt: 339, E(): 2.5e-09, (59.15% identity in 93 aa
                     overlap) and O33049|MLCB57.05c|ML2151 (174 aa), FASTA
                     scores: opt: 329, E(): 4e-09, (58.14% identity in 86 aa
                     overlap). Contains a possible secretory signal sequence in
                     N-terminus. Possible autocrine and/or paracrine bacterial
                     growth factor or cytokine (see citation below). Protein
                     product from Mb2477c detected using SWATH mass
                     spectrometry. Mb2477c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="InterPro:IPR010618"
                     /db_xref="InterPro:IPR023346"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y199"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MKNARTTLIA AAIAGTLVTT SPAGIANADD AGLDPNAAAG PDAVGFDPNL PPAPDAAPVD
       61 TPPAPEDAGF DPNLPPPLAP DFLSPPAEEA PPVPVAYSVN WDAIAQCESG GNWSINTGNG
      121 YYGGLQFTAG TWRANGGSGS AANASREEQI RVAENVLRSQ GIRAWPVCGR RG
//