LOCUS SIU01091.1 419 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 putative membrane protein protein. ACCESSION LT708304-2505 PROTEIN_ID SIU01091.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2476C" /note="Mb2476c, -, len: 419 aa. Equivalent to Rv2449c, len: 419 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 419 aa overlap). Conserved hypothetical protein, highly similar to hypothetical proteins e.g. P95139|Rv2953|MTCY349.37c from M. tuberculosis (418 aa), FASTA scores: opt: 1829, E(): 4.7e-103, (67.3% identity in 419 aa overlap); AAK47353|MT3027 from Mycobacterium tuberculosis strain CDC1551 (418 aa), FASTA score: opt: 1829, E(): 4.7e-103, (67.3 identity in 419 aa overlap); Q9CD87|ML0129 from Mycobacterium leprae (418 aa), FASTA scores: opt: 1727, E(): 6.8e-97, (65.45% identity in 414 aa overlap); etc. Protein product from Mb2476c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2476c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y194" /db_xref="InterPro:IPR005097" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3Y194" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTATPREFDI VLYGATGFVG KLTAEYLARA GGDARIALAG RSTQRVLAVR EALGESAQTW 61 PILTADASLP STLQAMAARA QVVVTTVGPY TRYGLPLVAA CAAAGTDYAD LTGEPMFMRN 121 SIDLYHKQAA DTGARIVHAC GFDSVPSDLS VYALYHAARE DGAGELTDTN CVVRSFKGGF 181 SGGTIASMLE VLSTASNDPD ARRQLSDPYM LSPDRGAEPE LGPQPDLPSR RGRRLAPELA 241 GVWTAGFIMA PTNTRIVRRS NALLDWAYGR RFRYSETMSV GSTVLAPVVS VVGGGVGNAM 301 FGLASRYIRL LPRGLVKRVV PKPGTGPSAA ARERGYYRIE TYTTTTTGAR YLARMAQDGD 361 PGYKATSVLL GECGLALALD RDKLSDMRGV LTPAAAMGDA LLERLPAAGV SLQTTRLAS //