LOCUS       SIU01079.1               679 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 GLUTAMINE-
            DEPENDENT NAD(+) SYNTHETASE NADE (NAD(+) SYNTHASE [GLUTAMINE-
            HYDROLYSING]) protein.
ACCESSION   LT708304-2493
PROTEIN_ID  SIU01079.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="nadE"
                     /locus_tag="BQ2027_MB2464C"
                     /note="Mb2464c, nadE, len: 679 aa. Equivalent to Rv2438c,
                     len: 679 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 679 aa overlap). nadE,
                     glutamine-dependent NAD(+) synthetase (EC 6.3.5.1) (see
                     citation below), equivalent to Q9CBZ6|NADE_MYCLE|ML1463
                     Glutamine-dependent NAD(+) synthetase from Mycobacterium
                     leprae (680 aa), FASTA scores: opt: 3877, E(): 0. Also
                     similar to others e.g. O83759|NADE_TREPA|TP0780 from
                     Treponema pallidum (679 aa), FASTA scores: opt: 543, E():
                     1.1e-25; O74940|NADE_SCHPO|SPCC553.02 from
                     Schizosaccharomyces pombe (Fission yeast) (700 aa), FASTA
                     scores: opt: 354, E(): 4.7e-14; P38795|NADE_YEAST|YHR074W
                     from Saccharomyces cerevisiae (Baker's yeast) (714 aa),
                     FASTA scores: opt: 339, E(): 4e-13; etc. Contains PS00591
                     Glycosyl hydrolases family 10 active site. BELONGS TO THE
                     NAD SYNTHETASE FAMILY IN THE C-TERMINAL SECTION.
                     N-terminus shorter since first submission. Protein product
                     from Mb2464c detected using shotgun mass spectrometry and
                     SWATH mass spectrometry. Mb2464c found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P0A5L7"
                     /db_xref="InterPro:IPR003010"
                     /db_xref="InterPro:IPR003694"
                     /db_xref="InterPro:IPR014445"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="InterPro:IPR022310"
                     /db_xref="InterPro:IPR036526"
                     /db_xref="InterPro:IPR041856"
                     /db_xref="UniProtKB/Swiss-Prot:P0A5L7"
BEGIN
        1 MNFYSAYQHG FVRVAACTHH TTIGDPAANA ASVLDMARAC HDDGAALAVF PELTLSGYSI
       61 EDVLLQDSLL DAVEDALLDL VTESADLLPV LVVGAPLRHR HRIYNTAVVI HRGAVLGVVP
      121 KSYLPTYREF YERRQMAPGD GERGTIRIGG ADVAFGTDLL FAASDLPGFV LHVEICEDMF
      181 VPMPPSAEAA LAGATVLANL SGSPITIGRA EDRRLLARSA SARCLAAYVY AAAGEGESTT
      241 DLAWDGQTMI WENGALLAES ERFPKGVRRS VADVDTELLR SERLRMGTFD DNRRHHRELT
      301 ESFRRIDFAL DPPAGDIGLL REVERFPFVP ADPQRLQQDC YEAYNIQVSG LEQRLRALDY
      361 PKVVIGVSGG LDSTHALIVA THAMDREGRP RSDILAFALP GFATGEHTKN NAIKLARALG
      421 VTFSEIDIGD TARLMLHTIG HPYSVGEKVY DVTFENVQAG LRTDYLFRIA NQRGGIVLGT
      481 GDLSELALGW STYGVGDQMS HYNVNAGVPK TLIQHLIRWV ISAGEFGEKV GEVLQSVLDT
      541 EITPELIPTG EEELQSSEAK VGPFALQDFS LFQVLRYGFR PSKIAFLAWH AWNDAERGNW
      601 PPGFPKSERP SYSLAEIRHW LQIFVQRFYS FSQFKRSALP NGPKVSHGGA LSPRGDWRAP
      661 SDMSARIWLD QIDREVPKG
//