LOCUS SIU01042.1 653 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE GTP-BINDING PROTEIN LEPA (GTP-BINDING ELONGATION FACTOR) protein. ACCESSION LT708304-2456 PROTEIN_ID SIU01042.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="lepA" /locus_tag="BQ2027_MB2427C" /note="Mb2427c, lepA, len: 653 aa. Equivalent to Rv2404c, len: 653 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 653 aa overlap). Probable lepA, GTP-binding protein (a protein of unknown function, but apparently with membrane-related functions and very similar to protein synthesis elongation factors; see citations below). Equivalent to P53530|LEPA_MYCLE|ML0611|B1937_F3_81 GTP-BINDING PROTEIN from Mycobacterium leprae (646 aa), FASTA scores: opt: 3610, E(): 1.2e-205, (88.0% identity in 649 aa overlap). Also highly similar to many GTP-BINDING PROTEINS LEPA e.g. Q9RDC9|LEPA_STRCO|SCC77.29c from Streptomyces coelicolor (622 aa), FASTA scores: opt: 3046, E(): 2.3e-172, (74.3% identity in 626 aa overlap); P37949|LEPA_BACSU from B. subtilis (612 aa), FASTA scores: opt: 2430, E(): 5.3e-136, (58.7% identity in 610 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00301 GTP-binding elongation factors signature. BELONGS TO THE GTP-BINDING ELONGATION FACTOR FAMILY, LEPA SUBFAMILY. Protein product from Mb2427c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2427c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65270" /db_xref="InterPro:IPR000640" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR006297" /db_xref="InterPro:IPR009000" /db_xref="InterPro:IPR013842" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR031157" /db_xref="InterPro:IPR035647" /db_xref="InterPro:IPR035654" /db_xref="InterPro:IPR038363" /db_xref="UniProtKB/Swiss-Prot:P65270" BEGIN 1 MRTPCSQHRR DRPSAIGSQL PDADTLDTRQ PPLQEIPISS FADKTFTAPA QIRNFCIIAH 61 IDHGKSTLAD RMLQLTGVVD ERSMRAQYLD RMDIERERGI TIKAQNVRLP WRVDKTDYVL 121 HLIDTPGHVD FTYEVSRALE ACEGAVLLVD AAQGIEAQTL ANLYLALDRD LHIIPVLNKI 181 DLPAADPDRY AAEMAHIIGC EPAEVLRVSG KTGEGVSDLL DEVVRQVPPP QGDAEAPTRA 241 MIFDSVYDIY RGVVTYVRVV DGKISPRERI MMMSTGATHE LLEVGIVSPE PKPCEGLGVG 301 EVGYLITGVK DVRQSKVGDT VTSLSRARGA AAEALTGYRE PKPMVYSGLY PVDGSDYPNL 361 RDALDKLQLN DAALTYEPET SVALGFGFRC GFLGLLHMEI TRERLEREFG LDLISTSPNV 421 VYRVHKDDGT EIRVTNPSDW PEGKIRTVYE PVVKTTIIAP SEFIGTIMEL CQSRRGELGG 481 MDYLSPERVE LRYTMPLGEI IFDFFDALKS RTRGYASLDY EEAGEQEAAL VKVDILLQGE 541 AVDAFSAIVH KDTAYAYGNK MTTKLKELIP RQQFEVPVQA AIGSKIIARE NIRAIRKDVL 601 SKCYGGDITR KRKLLEKQKE GKKRMKTIGR VEVPQEAFVA ALSTDAAGDK GKK //