LOCUS SIU01037.1 356 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SULFATE-BINDING LIPOPROTEIN SUBI protein. ACCESSION LT708304-2451 PROTEIN_ID SIU01037.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="subI" /locus_tag="BQ2027_MB2422C" /note="Mb2422c, subI, len: 356 aa. Equivalent to Rv2400c, len: 356 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 356 aa overlap). Probable subI, sulfate-binding lipoprotein component of sulfate transport system (see citations below), equivalent to Q9CCN3|SUBI|ML0615 (alias Q49748|B1937_F1_11, 358 aa) PUTATIVE SULPHATE-BINDING PROTEIN from Mycobacterium leprae (348 aa), FASTA scores: opt: 1775, E(): 2.3e-102, (76.45% identity in 340 aa overlap). Also similar to others and other substrate-binding proteins e.g. P27366|SUBI_SYNP7|SBPA SULFATE-BINDING PROTEIN PRECURSOR from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (350 aa), FASTA scores: opt: 703, E(): 4.6e-36, (35.6% identity in 351 aa overlap); Q9I6K7|SBP|PA0283 SULFATE-BINDING PROTEIN PRECURSOR from Pseudomonas aeruginosa (332 aa), FASTA scores: opt: 591, E(): 3.7e-29, (36.9% identity in 317 aa overlap); CAC49112|SMB21133 PUTATIVE SULFATE UPTAKE ABC TRANSPORTER PERIPLASMIC SOLUTE-BINDING PROTEIN PRECURSOR from Rhizobium meliloti (Sinorhizobium meliloti) (341 aa), FASTA scores: opt: 569, E(): 8.8e-28, (36.15% identity in 321 aa overlap); etc. BELONGS TO THE PROKARYOTIC SULFATE BINDING PROTEIN FAMILY. Protein product from Mb2422c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2422c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y1G6" /db_xref="InterPro:IPR005669" /db_xref="UniProtKB/TrEMBL:A0A1R3Y1G6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLSLTLSEAS CIASASRWRH IIPAGVVCAL IAGIGVGCHG GPSDVVGRAG PDRAHTSITL 61 VAYAVPEPGW SAVIPAFNAS EQGRGVQVIT SYGASADQSR GVADGKPADL VNFSVEPDIA 121 RLVKAGKVDK DWDADATKGI PFGSVVTFVV RAGNPKNIRD WDDLLRPGIE VITPSPLSSG 181 SAKWNLLAPY AAKSDGGRNN QAGIDFVNTL VNEHVKLRPG SGREATDVFV QGSGDVLISY 241 ENEAIATERA GKPVQHVTPP QTFKIENPLA VVATSTHLGA ATAFRNFQYT VQAQKLWAQA 301 GFRPVDPAVA ADFADLFPVP AKLWTIADLG GWGSVDPQLF DKATGSITKI YLRATG //