LOCUS       SIU01026.1               254 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            3'-phosphoadenosine 5'-phosphosulfate reductase cysh (
            paps reductase, thioredoxin dep.) (padops reductase) (
            3'-phosphoadenylylsulfate reductase) (paps sulfotransferase)
            protein.
ACCESSION   LT708304-2440
PROTEIN_ID  SIU01026.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cysH"
                     /locus_tag="BQ2027_MB2413"
                     /note="Mb2413, cysH, len: 254 aa. Equivalent to Rv2392,
                     len: 254 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 254 aa overlap). Probable cysH,
                     3'-phosphoadenosine 5'-phosphosulfate reductase (EC
                     1.8.4.8), similar to many e.g.
                     P94498|O34620|CYH1_BACSU|CYSH from Bacillus subtilis (233
                     aa), FASTA scores: opt: 618, E(): 8.1e-32, (46.5% identity
                     in 202 aa overlap); Q9KCT3|CYSH|BH1486 from Bacillus
                     halodurans (231 aa), FASTA scores: opt: 560, E(): 3.6e-28,
                     (41.3% identity in 230 aa overlap); P56860|CYSH_DEIRA from
                     Deinococcus radiodurans (255 aa), FASTA scores: opt: 489,
                     E(): 1.1e-23, (44.7% identity in 190 aa overlap); etc.
                     BELONGS TO THE PAPS REDUCTASE FAMILY and CYSH SUBFAMILY.
                     Note that operon cysA-cysW-cysT-subI, probably involved in
                     sulfate transport, is near this putative ORF. Protein
                     product from Mb2413 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb2413 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:P65669"
                     /db_xref="InterPro:IPR002500"
                     /db_xref="InterPro:IPR004511"
                     /db_xref="InterPro:IPR011798"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="UniProtKB/Swiss-Prot:P65669"
BEGIN
        1 MSGETTRLTE PQLRELAARG AAELDGATAT DMLRWTDETF GDIGGAGGGV SGHRGWTTCN
       61 YVVASNMADA VLVDLAAKVR PGVPVIFLDT GYHFVETIGT RDAIESVYDV RVLNVTPEHT
      121 VAEQDELLGK DLFARNPHEC CRLRKVVPLG KTLRGYSAWV TGLRRVDAPT RANAPLVSFD
      181 ETFKLVKVNP LAAWTDQDVQ EYIADNDVLV NPLVREGYPS IGCAPCTAKP AEGADPRSGR
      241 WQGLAKTECG LHAS
//