LOCUS SIU01026.1 254 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable 3'-phosphoadenosine 5'-phosphosulfate reductase cysh ( paps reductase, thioredoxin dep.) (padops reductase) ( 3'-phosphoadenylylsulfate reductase) (paps sulfotransferase) protein. ACCESSION LT708304-2440 PROTEIN_ID SIU01026.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cysH" /locus_tag="BQ2027_MB2413" /note="Mb2413, cysH, len: 254 aa. Equivalent to Rv2392, len: 254 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 254 aa overlap). Probable cysH, 3'-phosphoadenosine 5'-phosphosulfate reductase (EC 1.8.4.8), similar to many e.g. P94498|O34620|CYH1_BACSU|CYSH from Bacillus subtilis (233 aa), FASTA scores: opt: 618, E(): 8.1e-32, (46.5% identity in 202 aa overlap); Q9KCT3|CYSH|BH1486 from Bacillus halodurans (231 aa), FASTA scores: opt: 560, E(): 3.6e-28, (41.3% identity in 230 aa overlap); P56860|CYSH_DEIRA from Deinococcus radiodurans (255 aa), FASTA scores: opt: 489, E(): 1.1e-23, (44.7% identity in 190 aa overlap); etc. BELONGS TO THE PAPS REDUCTASE FAMILY and CYSH SUBFAMILY. Note that operon cysA-cysW-cysT-subI, probably involved in sulfate transport, is near this putative ORF. Protein product from Mb2413 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2413 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65669" /db_xref="InterPro:IPR002500" /db_xref="InterPro:IPR004511" /db_xref="InterPro:IPR011798" /db_xref="InterPro:IPR014729" /db_xref="UniProtKB/Swiss-Prot:P65669" BEGIN 1 MSGETTRLTE PQLRELAARG AAELDGATAT DMLRWTDETF GDIGGAGGGV SGHRGWTTCN 61 YVVASNMADA VLVDLAAKVR PGVPVIFLDT GYHFVETIGT RDAIESVYDV RVLNVTPEHT 121 VAEQDELLGK DLFARNPHEC CRLRKVVPLG KTLRGYSAWV TGLRRVDAPT RANAPLVSFD 181 ETFKLVKVNP LAAWTDQDVQ EYIADNDVLV NPLVREGYPS IGCAPCTAKP AEGADPRSGR 241 WQGLAKTECG LHAS //