LOCUS SIU01013.1 1682 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PEPTIDE
SYNTHETASE MBTE (PEPTIDE SYNTHASE) protein.
ACCESSION LT708304-2427
PROTEIN_ID SIU01013.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="mbtE"
/locus_tag="BQ2027_MB2401C"
/note="Mb2401c, mbtE, len: 1682 aa. Equivalent to Rv2380c,
len: 1682 aa, from Mycobacterium tuberculosis strain
H37Rv, (100.0% identity in 1682 aa overlap). mbtE, peptide
synthetase (see citations below), similar in part to
several synthases e.g. O07944|SNBDE PRISTINAMYCIN I
SYNTHASE 3 AND 4 from Streptomyces pristinaespiralis (4848
aa), FASTA scores: opt: 2635, E(): 1.9e-146, (36.8%
identity in 1657 aa overlap); O05647|SNBDE VIRGINIAMYCIN S
SYNTHETASE (FRAGMENT) from Streptomyces virginiae (1997
aa) FASTA scores: opt: 2580, E(): 1.6e-143, (40.65%
identity in 1163 aa overlap); Q9R9I2|DHBF PROTEIN INVOLVED
IN SIDEROPHORE PRODUCTION from Bacillus subtilis (2378
aa), FASTA scores: opt: 2388, E(): 3.6e-132, (33.9%
identity in 1579 aa overlap); O68487|ACMB ACTINOMYCIN
SYNTHETASE II from Streptomyces chrysomallus (2611 aa),
FASTA scores: opt: 2165, E(): 4.9e-119, (35.0% identity in
1634 aa overlap); etc. Equivalent to AAK46743 from
Mycobacterium tuberculosis strain CDC1551 (1787 aa) but
shorter 105 aa. Contains PS00455 putative AMP-binding
domain signature, and PS00012 Phosphopantetheine
attachment site. BELONGS TO THE ATP-DEPENDENT AMP-BINDING
ENZYME FAMILY. Protein product from Mb2401c detected using
shotgun mass spectrometry and SWATH mass spectrometry.
Mb2401c found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing."
/db_xref="GOA:A0A1R3Y0Z1"
/db_xref="InterPro:IPR000873"
/db_xref="InterPro:IPR001242"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR010071"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020845"
/db_xref="InterPro:IPR023213"
/db_xref="InterPro:IPR025110"
/db_xref="InterPro:IPR036736"
/db_xref="InterPro:IPR042099"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y0Z1"
BEGIN
1 MWFVQMADPS GALLNICVSY RITGDIDLAR LRDAVNAVAR RHRILRTTYP VGDDGVAQPT
61 VHADLRPGWT QYDLTDLSQR AQRLRLEVLA QREFCAPFEL SRDAPLRITV VRTAADEHVL
121 LLVAHHIAWD DGSWRVFFTD LTQAYSRADL GADLGPEHRP SAASGPDTTE ADLNYWRAIM
181 ADPPEPLELP GPAGTCVPTS WRAARATLRL PADTAARVAT MAKNTGCTPY MVLLAAFGAL
241 VHRYTHSDDF LVAAPVLNRG AGTEDAIGYF GNTVAMRLRP QSAMSFRELL TATRDIASGA
301 FAHQRINLDR VVRELNPDRR HGAERMTRVS FGFREPDGGG FNPPGIECER YDLRSNITQL
361 PLGFMVEFDR AGVLVEAEHL VEILEPALAK QMLRHFGVLL DNALAAPDNT LSGLALMDER
421 DAARLREVSR GERFDTPVKT LVDLVNEQTT RTPDATAVVY EGQHFTYHDL NEASNRLGHW
481 LIEQGIGSED RVAVLLDKSP DLIVTALGVV KSGAVYVPVD PSYPQDRLDF ILADCDAKLV
541 LRTPVRELAG YRSDDPTDAD RIRPLRPDNT AYLIYTSGTT GLPKGVAVPH RPVAEYFVWF
601 KGEYDVDDTD RLLQVASPSF DVSIAEIFGT LACGARMVIP RPGGLTDIGY LTALLRDEGI
661 TAMHFVPSLL GLFLSLPGVS QWRTLQRVPI GGEPLPGEVA DKFHATFDAL LHNFYGPTET
721 VINASRFKVV GPQGTRIVPI GRPKINTTMH LLDDSLQPVP TGVIGEIYIG GTHVAYGYHR
781 RAGLTAERFV ADPFNPGSRM YRSGDLARRN ADGDIEFVGR ADEQVKIRGF RIELGDVAAA
841 IAVDPTVGQA VVVVSDLPRL GKSLVGYVTP AAGGDGPADV GVDLDRIRAR VAAALPEYML
901 PAAYVVLDEI PITAHGKIDR AALPEPQIAS DTEFRAPQTA TERRLAQLFG ELLGRDRVGA
961 DDSFFDLGGH SLLATKLVAA VRNAFGVDVG VREIFEFATV TALAGHIDTL DSDSARPRLT
1021 RVDHDGPVRL SSSQMRSWFN YRFDGPNAVN NIPFAAALHG PCDTNAFAAA ITDVVARHEI
1081 LRTVYREIGG VPHQIIQPPA EVPVRCAAGS DAAWLRAELN NERGYVFDLE TDWPIRAALL
1141 STPEQTVLSL VVHHIAGDHW SAGVLFTDLL TAYRARSTGQ RPSWAPLPVQ YADYSVWQSA
1201 LLDDGAGIVG PQRDYWIRQL GGLAGETGLR PDFPRPALLS GAGDAVEFRL GAAIRDKLAA
1261 VSRDLGVTEF MLLQAAVAVV LHKAGGGVDV PIGAPVAGRS EANLDQLIGF FINIVVLRND
1321 LRGNPTLREV LQRTRQMALA AYAHQDLPFD QVVEAVNPQR SLSRNPLFDI VVHVREQMPQ
1381 DHVIDTGPDG DTTLRVLEPT FDAAQADLSV NFFACGDEYR GHVIYRTELY ERATAQRFAD
1441 WLVRVVEAFA DRPDQPLREV EMVSAQARRR ILDRSNAGAG TARVYLLDDA LKPVPVGVVG
1501 DVYYGGGPAV GARLARPSET ATRFVADPFA AQPGSRLYRN GERGVWKADG QLELLAEIER
1561 LPTAQAAPVP AEPADTETER ALAAILADVL EVGEVGRYDD FFNLGGDSIL ATQVAARARD
1621 GGIPLTARMV FEHPVLCELA AAVDAKPHVE AEPDDKHHAP MSTSGLSPDE LSALTASWDQ
1681 WP
//