LOCUS SIU01006.1 382 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CHAPERONE PROTEIN DNAJ2 protein. ACCESSION LT708304-2420 PROTEIN_ID SIU01006.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="dnaJ2" /locus_tag="BQ2027_MB2394C" /note="Mb2394c, dnaJ2, len: 382 aa. Equivalent to Rv2373c, len: 382 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 382 aa overlap). Probable dnaJ2, chaperone protein, equivalent to Q49762|DNJ2_MYCLE|ML0625|B1937_F2_56 CHAPERONE PROTEIN from Mycobacterium leprae (378 aa), FASTA scores: opt: 2301, E(): 1.7e-120, (87.5% identity in 382 aa overlap). Also highly similar to OTHER CHAPERONE PROTEINS DNAJ/DNAJ2 e.g. Q9RDD7|DNJ2_STRCO|SCC77.21c from Streptomyces coelicolor (378 aa), FASTA scores: opt: 1456, E(): 1.2e-73, (54.8% identity in 385 aa overlap); O52164|DNJ2_STRAL from Streptomyces albus (379 aa) FASTA scores: opt: 1378, E(): 2.6e-69, (52.2% identity in 385 aa overlap); Q9S5A3|DNAJ_LISMO from Listeria monocytogenes (377 aa), FASTA scores: opt: 1013, E(): 4.6e-49, (41.3% identity in 385 aa overlap); etc. Also similar to Rv0352|MTCY13E10.12 from Mycobacterium tuberculosis. Contains 1 J domain and 1 CR domain. BELONGS TO THE DNAJ FAMILY. Protein product from Mb2394c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2394c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P63967" /db_xref="InterPro:IPR001305" /db_xref="InterPro:IPR001623" /db_xref="InterPro:IPR002939" /db_xref="InterPro:IPR008971" /db_xref="InterPro:IPR012724" /db_xref="InterPro:IPR036410" /db_xref="InterPro:IPR036869" /db_xref="UniProtKB/Swiss-Prot:P63967" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MARDYYGLLG VSKNASDADI KRAYRKLARE LHPDVNPDEA AQAKFKEISV AYEVLSDPDK 61 RRIVDLGGDP LESAAAGGNG FGGFGGLGDV FEAFFGGGFG GGAASRGPIG RVRPGSDSLL 121 RMRLDLEECA TGVTKQVTVD TAVLCDRCQG KGTNGDSVPI PCDTCGGRGE VQTVQRSLLG 181 QMLTSRPCPT CRGVGVVIPD PCQQCMGDGR IRARREISVK IPAGVGDGMR VRLAAQGEVG 241 PGGGPAGDLY VEVHEQAHDV FVREGDHLHC TVSVPMVDAA LGVTVTVDAI LDGLSEITIP 301 PGTQPGSVIT LRGRGMPHLR SNTRGDLHVH VEVVVPTRLD HQDIELLREL KGRRDREVAE 361 VRSTHAAAGG LFSRLRETFT GR //