LOCUS SIU00974.1 310 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 cysteine synthase a cysk1 (o-acetylserine sulfhydrylase a) (o-acetylserine (thiol)-lyase a) (csase a) protein. ACCESSION LT708304-2388 PROTEIN_ID SIU00974.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cysK1" /locus_tag="BQ2027_MB2362" /note="Mb2362, cysK1, len: 310 aa. Equivalent to Rv2334, len: 310 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 310 aa overlap). Probable cysK1, cysteine synthase A (EC 4.2.99.8), equivalent to O32978|CYSK_MYCLE|ML0839|MLCB22.47 CYSTEINE SYNTHASE A from Mycobacterium leprae (310 aa), FASTA scores: opt: 1756, E(): 8.6e-96, (85.8% identity in 310 aa overlap). Also highly similar to other CYSTEINE SYNTHASES e.g. Q9JQL6|CYSK|NMA0974|NMB0763 PUTATIVE CYSTEINE SYNTHASE from Neisseria meningitidis (serogroup A and B) (310 aa), FASTA scores: opt: 1368, E(): 4.6e-73, (66.45% identity in 310 aa overlap); P73410|CYSK_SYNY3|SLR1842 from Synechocystis sp (312 aa), FASTA scores: opt: 1310, E(): 1.2e-69, (64.65% identity in 311 aa overlap); Q43725|CYSM_ARATH|OASC|ACS1|AT3G59760|F24G16.30 CYSTEINE SYNTHASE (MITOCHONDRIAL PRECURSOR) from Arabidopsis thaliana (Mouse-ear cress) (424 aa), FASTA scores: opt: 1253, E(): 3.2e-66, (59.2% identity in 309 aa overlap) (has its N-terminus longer 104 aa); etc. Contains PS00901 Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. BELONGS TO THE CYSTEINE SYNTHASE/CYSTATHIONINE BETA-SYNTHASE FAMILY. Note that previously known as cysK. Protein product from Mb2362 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2362 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A535" /db_xref="InterPro:IPR001216" /db_xref="InterPro:IPR001926" /db_xref="InterPro:IPR005856" /db_xref="InterPro:IPR005859" /db_xref="InterPro:IPR036052" /db_xref="UniProtKB/Swiss-Prot:P0A535" BEGIN 1 MSIAEDITQL IGRTPLVRLR RVTDGAVADI VAKLEFFNPA NSVKDRIGVA MLQAAEQAGL 61 IKPDTIILEP TSGNTGIALA MVCAARGYRC VLTMPETMSL ERRMLLRAYG AELILTPGAD 121 GMSGAIAKAE ELAKTDQRYF VPQQFENPAN PAIHRVTTAE EVWRDTDGKV DIVVAGVGTG 181 GTITGVAQVI KERKPSARFV AVEPAASPVL SGGQKGPHPI QGIGAGFVPP VLDQDLVDEI 241 ITVGNEDALN VARRLAREEG LLVGISSGAA TVAALQVARR PENAGKLIVV VLPDFGERYL 301 STPLFADVAD //