LOCUS       SIU00974.1               310 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 cysteine
            synthase a cysk1 (o-acetylserine sulfhydrylase a) (o-acetylserine
            (thiol)-lyase a) (csase a) protein.
ACCESSION   LT708304-2388
PROTEIN_ID  SIU00974.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cysK1"
                     /locus_tag="BQ2027_MB2362"
                     /note="Mb2362, cysK1, len: 310 aa. Equivalent to Rv2334,
                     len: 310 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 310 aa overlap). Probable cysK1,
                     cysteine synthase A (EC 4.2.99.8), equivalent to
                     O32978|CYSK_MYCLE|ML0839|MLCB22.47 CYSTEINE SYNTHASE A
                     from Mycobacterium leprae (310 aa), FASTA scores: opt:
                     1756, E(): 8.6e-96, (85.8% identity in 310 aa overlap).
                     Also highly similar to other CYSTEINE SYNTHASES e.g.
                     Q9JQL6|CYSK|NMA0974|NMB0763 PUTATIVE CYSTEINE SYNTHASE
                     from Neisseria meningitidis (serogroup A and B) (310 aa),
                     FASTA scores: opt: 1368, E(): 4.6e-73, (66.45% identity in
                     310 aa overlap); P73410|CYSK_SYNY3|SLR1842 from
                     Synechocystis sp (312 aa), FASTA scores: opt: 1310, E():
                     1.2e-69, (64.65% identity in 311 aa overlap);
                     Q43725|CYSM_ARATH|OASC|ACS1|AT3G59760|F24G16.30 CYSTEINE
                     SYNTHASE (MITOCHONDRIAL PRECURSOR) from Arabidopsis
                     thaliana (Mouse-ear cress) (424 aa), FASTA scores: opt:
                     1253, E(): 3.2e-66, (59.2% identity in 309 aa overlap)
                     (has its N-terminus longer 104 aa); etc. Contains PS00901
                     Cysteine synthase/cystathionine beta-synthase P-phosphate
                     attachment site. BELONGS TO THE CYSTEINE
                     SYNTHASE/CYSTATHIONINE BETA-SYNTHASE FAMILY. Note that
                     previously known as cysK. Protein product from Mb2362
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb2362 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P0A535"
                     /db_xref="InterPro:IPR001216"
                     /db_xref="InterPro:IPR001926"
                     /db_xref="InterPro:IPR005856"
                     /db_xref="InterPro:IPR005859"
                     /db_xref="InterPro:IPR036052"
                     /db_xref="UniProtKB/Swiss-Prot:P0A535"
BEGIN
        1 MSIAEDITQL IGRTPLVRLR RVTDGAVADI VAKLEFFNPA NSVKDRIGVA MLQAAEQAGL
       61 IKPDTIILEP TSGNTGIALA MVCAARGYRC VLTMPETMSL ERRMLLRAYG AELILTPGAD
      121 GMSGAIAKAE ELAKTDQRYF VPQQFENPAN PAIHRVTTAE EVWRDTDGKV DIVVAGVGTG
      181 GTITGVAQVI KERKPSARFV AVEPAASPVL SGGQKGPHPI QGIGAGFVPP VLDQDLVDEI
      241 ITVGNEDALN VARRLAREEG LLVGISSGAA TVAALQVARR PENAGKLIVV VLPDFGERYL
      301 STPLFADVAD
//