LOCUS       SIU00935.1               230 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            CUTINASE CUT2 protein.
ACCESSION   LT708304-2349
PROTEIN_ID  SIU00935.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cut2"
                     /locus_tag="BQ2027_MB2323"
                     /standard_name="cfp25"
                     /note="Mb2323, cut2, len: 230 aa. Equivalent to Rv2301,
                     len: 230 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 230 aa overlap). Probable cut2
                     (alternate gene name: cfp25), cutinase (EC 3.1.1.-),
                     highly similar to others from Mycobacteria tuberculosis
                     e.g. MTCY13E12.04|Rv3451|O06318|CUT3_MYCTU (247 aa), FASTA
                     scores: opt: 569, E(): 2.3e-27, (45.3% identity in 223 aa
                     overlap); MT2037|MTCY39.35|RV1984C|Q10837|CUT1_MYCTU (217
                     aa), FASTA scores: opt: 383, E(): 3.4e-16 (42.9% identity
                     in 217 aa overlap); O69691|Rv3724|MTV025.072 PUTATIVE
                     CUTINASE PRECURSOR (187 aa), FASTA scores: opt: 248, E():
                     4.3e-08, (41.85% identity in 172 aa overlap); etc. Also
                     similar to few others from other organisms e.g. Q9KK87
                     SERINE ESTERASE CUTINASE from Mycobacterium avium (220
                     aa), FASTA scores: opt: 391, E(): 1.1e-16, (39.15%
                     identity in 235 aa overlap); etc. Contains PS00095 C-5
                     cytosine-specific DNA methylases C-terminal signature.
                     BELONGS TO THE CUTINASE FAMILY. Start changed since first
                     submission (+11 aa). Protein product from Mb2323 detected
                     using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb2323 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P63882"
                     /db_xref="InterPro:IPR000675"
                     /db_xref="InterPro:IPR006311"
                     /db_xref="InterPro:IPR011150"
                     /db_xref="InterPro:IPR029058"
                     /db_xref="UniProtKB/Swiss-Prot:P63882"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MNDLLTRRLL TMGAAAAMLA AVLLLTPITV PAGYPGAVAP ATAACPDAEV VFARGRFEPP
       61 GIGTVGNAFV SALRSKVNKN VGVYAVKYPA DNQIDVGAND MSAHIQSMAN SCPNTRLVPG
      121 GYSLGAAVTD VVLAVPTQMW GFTNPLPPGS DEHIAAVALF GNGSQWVGPI TNFSPAYNDR
      181 TIELCHGDDP VCHPADPNTW EANWPQHLAG AYVSSGMVNQ AADFVAGKLQ
//