LOCUS SIU00935.1 230 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CUTINASE CUT2 protein. ACCESSION LT708304-2349 PROTEIN_ID SIU00935.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cut2" /locus_tag="BQ2027_MB2323" /standard_name="cfp25" /note="Mb2323, cut2, len: 230 aa. Equivalent to Rv2301, len: 230 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 230 aa overlap). Probable cut2 (alternate gene name: cfp25), cutinase (EC 3.1.1.-), highly similar to others from Mycobacteria tuberculosis e.g. MTCY13E12.04|Rv3451|O06318|CUT3_MYCTU (247 aa), FASTA scores: opt: 569, E(): 2.3e-27, (45.3% identity in 223 aa overlap); MT2037|MTCY39.35|RV1984C|Q10837|CUT1_MYCTU (217 aa), FASTA scores: opt: 383, E(): 3.4e-16 (42.9% identity in 217 aa overlap); O69691|Rv3724|MTV025.072 PUTATIVE CUTINASE PRECURSOR (187 aa), FASTA scores: opt: 248, E(): 4.3e-08, (41.85% identity in 172 aa overlap); etc. Also similar to few others from other organisms e.g. Q9KK87 SERINE ESTERASE CUTINASE from Mycobacterium avium (220 aa), FASTA scores: opt: 391, E(): 1.1e-16, (39.15% identity in 235 aa overlap); etc. Contains PS00095 C-5 cytosine-specific DNA methylases C-terminal signature. BELONGS TO THE CUTINASE FAMILY. Start changed since first submission (+11 aa). Protein product from Mb2323 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2323 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P63882" /db_xref="InterPro:IPR000675" /db_xref="InterPro:IPR006311" /db_xref="InterPro:IPR011150" /db_xref="InterPro:IPR029058" /db_xref="UniProtKB/Swiss-Prot:P63882" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNDLLTRRLL TMGAAAAMLA AVLLLTPITV PAGYPGAVAP ATAACPDAEV VFARGRFEPP 61 GIGTVGNAFV SALRSKVNKN VGVYAVKYPA DNQIDVGAND MSAHIQSMAN SCPNTRLVPG 121 GYSLGAAVTD VVLAVPTQMW GFTNPLPPGS DEHIAAVALF GNGSQWVGPI TNFSPAYNDR 181 TIELCHGDDP VCHPADPNTW EANWPQHLAG AYVSSGMVNQ AADFVAGKLQ //