LOCUS SIU00911.1 396 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Cytochrome P450 121 CYP121 protein. ACCESSION LT708304-2325 PROTEIN_ID SIU00911.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cyp121" /locus_tag="BQ2027_MB2299" /note="Mb2299, cyp121, len: 396 aa. Equivalent to Rv2276, len: 396 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 396 aa overlap). cyp121, cytochrome P450 (EC 1.14.-.-) (see citation below), similar to e.g. G303644 (397 aa) opt: 675, z-score: 776.4, E(): 2.7e-36, (33.7% identity in 407 aa overlap); contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature, similar to MTCY339.42, 29.2% identity in 298 aa overlap. Protein product from Mb2299 detected using SWATH mass spectrometry. Mb2299 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A515" /db_xref="InterPro:IPR001128" /db_xref="InterPro:IPR002397" /db_xref="InterPro:IPR017972" /db_xref="InterPro:IPR036396" /db_xref="PDB:5EDT" /db_xref="UniProtKB/Swiss-Prot:P0A515" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTATVLLEVP FSARGDRIPD AVAELRTREP IRKVRTITGA EAWLVSSYAL CTQVLEDRRF 61 SMKETAAAGA PRLNALTVPP EVVNNMGNIA DAGLRKAVMK AITPKAPGLE QFLRDTANSL 121 LDNLITEGAP ADLRNDFADP LATALHCKVL GIPQEDGPKL FRSLSIAFMS SADPIPAAKI 181 NWDRDIEYMA GILENPNITT GLMGELSRLR KDPAYSHVSD ELFATIGVTF FGAGVISTGS 241 FLTTALISLI QRPQLRNLLH EKPELIPAGV EELLRINLSF ADGLPRLATA DIQVGDVLVR 301 KGELVLVLLE GANFDPEHFP NPGSIELDRP NPTSHLAFGR GQHFCPGSAL GRRHAQIGIE 361 ALLKKMPGVD LAVPIDQLVW RTRFQRRIPE RLPVLW //