LOCUS SIU00896.1 502 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Apolipoprotein N-acyltransferase protein. ACCESSION LT708304-2310 PROTEIN_ID SIU00896.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2285C" /note="Mb2285c, -, len: 502 aa. Equivalent to Rv2262c and Rv2261c, len: 360 aa and 140 aa, from Mycobacterium tuberculosis strain H37Rv, (94.7% identity in 357 aa overlap and 100.0% identity in 140 aa overlap). Conserved hypothetical protein, with function unknown but some similarity to N-terminal 70% of P23930|P77703|LNT_ECOLI|CUTE|B0657 APOLIPOPROTEIN N-ACYLTRANSFERASE (EC 2.3.1.-) from Escherichia coli strain K12 (512 aa), FASTA scores: opt: 239, E(): 1.6e-07, (30.4% identity in 359 aa overlap). Note that neighboring ORF shows similarity to N -terminal part of PCC6803 apolipoprotein N-acyltransferase from Synechocystis sp., suggesting possibility of frameshift. Sequence of clones from two sources has been checked but no error found. Appear to be two extra bases at position 1876970 compared to CDC1551 strain. Conserved hypothetical protein, with function unknown but some similarity to C-terminal end of PCC6803 apolipoprotein N-acyltransferase from Synechocystis sp. Note that next ORF shows similarity to N-terminal part of P74055 APOLIPOPROTEIN N-ACYLTRANSFERASE from Escherichia coli (519 aa), FASTA scores: opt: 142, E(): 0.007, (29.9% identity in 117 aa overlap), suggesting possible frameshift. Sequence of clones from two sources has been checked but no error found. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv2262c and Rv2261c exist as 2 genes. In Mycobacterium bovis, a 2 bp deletion (ct-*) results in a single product which is more similar to Rv2262c." /db_xref="GOA:A0A1R3Y0V4" /db_xref="InterPro:IPR003010" /db_xref="InterPro:IPR004563" /db_xref="InterPro:IPR036526" /db_xref="UniProtKB/TrEMBL:A0A1R3Y0V4" BEGIN 1 MALRAGARRQ PVIGCAAALV FGGLPALAFP APSWWWLAWF GLVPLLLVVR AAPTSWEGAL 61 RAWTGMGGFV LATQYWLVTS AGPMLVLLAA GLGVLWLPAG WLAHRLLSVP VTTCRVGAAL 121 VVVPSAWVAA EAVRSWQSLG GPWALLGASQ WSQPVTLASA SLGGVWLTSF LLVATNTAIA 181 SVLVCRATGG RLVALGCVIG CAGLGPASYL LGSVPVGGPT VRVALVQAGD IADAAARLAA 241 GEEFTAAVAD QRPDLVVWGE SSVGQDLTRH PDVLARLAEL SQRVGADLLV NVDAPAPDGG 301 IYKSAVLVGA HEAVGSYRKT RLVPFGEYVP LRPLFGWITR YSKAAAKDRQ RGAGPVVLAV 361 NSLHIAPLIS YEMTFSDLTR HAARLGAALL VYQSSTSTFQ GSWAQPQLAA QPAVRAVEAG 421 IPAVHASLSG DSSAFDTRGR RLAWCSAEFN GAIVVNVPLA SNVTLYLRLG DWVPVTAFVV 481 MGAGFAVFLR RSLARVSDCA DK //