LOCUS SIU00894.1 361 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 s-nitrosomycothio l reductase mscr protein. ACCESSION LT708304-2308 PROTEIN_ID SIU00894.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mscr" /locus_tag="BQ2027_MB2283" /note="Mb2283, adhE2, len: 361 aa. Equivalent to Rv2259, len: 361 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 361 aa overlap). Probable adhE2, zinc-containing alcohol dehydrogenase, similar to several, especially mycothiol-dependent formaldehyde dehydrogenase from Amycolatopsis methanolica P80094 (360 aa). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. FASTA scores: >sp|P80094|FADH_AMYME NAD/MYCOTHIOL-DEPENDENT FORMALDEHYDE DEHYDROGENASE (MD-FALDH) Length = 360, Expect = e-156, Identities = 268/358 (74%). Also similar to Rv0162c, (MTCI28.02c, 35.0% identity in 371 aa overlap). Protein product from Mb2283 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2283 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y2U2" /db_xref="InterPro:IPR002328" /db_xref="InterPro:IPR011032" /db_xref="InterPro:IPR013149" /db_xref="InterPro:IPR013154" /db_xref="InterPro:IPR017816" /db_xref="InterPro:IPR020843" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2U2" BEGIN 1 MSQTVRGVIA RQKGEPVELV NIVVPDPGPG EAVVDVTACG VCHTDLTYRE GGINDEYPFL 61 LGHEAAGIIE AVGPGVTAVE PGDFVILNWR AVCGQCRACK RGRPRYCFDT FNAEQKMTLT 121 DGTELTAALG IGAFADKTLV HSGQCTKVDP AADPAVAGLL GCGVMAGLGA AINTGGVTRD 181 DTVAVIGCGG VGDAAIAGAA LVGAKRIIAV DTDDTKLDWA RTFGATHTVN AREVDVVQAI 241 GGLTDGFGAD VVIDAVGRPE TYQQAFYARD LAGTVVLVGV PTPDMRLDMP LVDFFSHGGA 301 LKSSWYGDCL PESDFPTLID LYLQGRLPLQ RFVSERIGLE DVEEAFHKMH GGKVLRSVVM 361 L //