LOCUS SIU00880.1 416 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 3-OXOACYL-
[ACYL-CARRIER PROTEIN] SYNTHASE 1 KASA (BETA-KETOACYL-
ACP SYNTHASE) (KAS I) protein.
ACCESSION LT708304-2294
PROTEIN_ID SIU00880.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="kasA"
/locus_tag="BQ2027_MB2269"
/note="Mb2269, kasA, len: 416 aa. Equivalent to Rv2245,
len: 416 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 416 aa overlap). kasA,
beta-ketoacyl-ACP synthase (EC 2.3.1.41), involved in
meromycolate extension (see citations below): belongs to
the FAS-II system, which utilizes primarily palmitoyl-ACP
rather than short-chain acyl-ACP primers. Highly similar
to others e.g. L43074|STMFABD3|g870805 beta-ketoacyl-ACP
synthase from Streptomyces glaucescens (423 aa), FASTA
scores: opt: 1105, E(): 0, (44.6% identity in 417 aa
overlap); FABF_ECOLI|P39435
3-oxoacyl-[acyl-carrier-protein] synthase II from
Escherichia coli, FASTA score: (39.4% identity in 254 aa
overlap); FABB_HORVU|P23902
3-oxoacyl-[acyl-carrier-protein] synthase I, FASTA score:
(33.4% identity in 413 aa overlap); etc. Strongest
similarity to downstream ORF kasB|Rv2246|MTCY427.27
3-oxoacyl-[acyl-carrier-protein] synthase 2 from
Mycobacterium tuberculosis (438 aa), FASTA score: (66.3%
identity in 409 aa overlap). BELONGS TO THE
BETA-KETOACYL-ACP SYNTHASES FAMILY. Protein product from
Mb2269 detected using shotgun mass spectrometry and SWATH
mass spectrometry. Mb2269 found to be expressed during
exponential growth in Sauton's minimal media by
RNA-sequencing."
/db_xref="GOA:P63455"
/db_xref="InterPro:IPR000794"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR020841"
/db_xref="UniProtKB/Swiss-Prot:P63455"
/experiment="experimental evidence, no additional details
recorded"
BEGIN
1 MSQPSTANGG FPSVVVTAVT ATTSISPDIE STWKGLLAGE SGIHALEDEF VTKWDLAVKI
61 GGHLKDPVDS HMGRLDMRRM SYVQRMGKLL GGQLWESAGS PEVDPDRFAV VVGTGLGGAE
121 RIVESYDLMN AGGPRKVSPL AVQMIMPNGA AAVIGLQLGA RAGVMTPVSA CSSGSEAIAH
181 AWRQIVMGDA DVAVCGGVEG PIEALPIAAF SMMRAMSTRN DEPERASRPF DKDRDGFVFG
241 EAGALMLIET EEHAKARGAK PLARLLGAGI TSDAFHMVAP AADGVRAGRA MTRSLELAGL
301 SPADIDHVNA HGTATPIGDA AEANAIRVAG CDQAAVYAPK SALGHSIGAV GALESVLTVL
361 TLRDGVIPPT LNYETPDPEI DLDVVAGEPR YGDYRYAVNN SFGFGGHNVA LAFGRY
//