LOCUS SIU00861.1 364 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 multifunctional protein. has rnase h,alpha-ribazole phosphatase, and acid phosphatase activities. protein. ACCESSION LT708304-2275 PROTEIN_ID SIU00861.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2253C" /note="Mb2253c, -, len: 364 aa. Equivalent to Rv2228c, len: 364 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 364 aa overlap). Conserved hypothetical protein. Some similarity to phosphoglycerate mutase and ribonuclease H. Similar to CAB88177.1|AL352972 putative bifunctional protein (ribonuclease H/phosphoglycerate mutase) from Streptomyces coelicolor A3(2) (497 aa); Smith-Waterman scores: 107 bits (424), Expect = 4e-41 Identities = 160/485 (32%). Also similar in C-terminal part to Rv2419c and Rv2135c. Protein product from Mb2253c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2253c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P64956" /db_xref="InterPro:IPR002156" /db_xref="InterPro:IPR012337" /db_xref="InterPro:IPR013078" /db_xref="InterPro:IPR014636" /db_xref="InterPro:IPR029033" /db_xref="InterPro:IPR036397" /db_xref="UniProtKB/Swiss-Prot:P64956" BEGIN 1 MKVVIEADGG SRGNPGPAGY GAVVWTADHS TVLAESKQAI GRATNNVAEY RGLIAGLDDA 61 VKLGATEAAV LMDSKLVVEQ MSGRWKVKHP DLLKLYVQAQ ALASQFRRIN YEWVPRARNT 121 YADRLANDAM DAAAQSAAAD ADPAKIVATE SPTSPGWTGA RGTPTRLLLL RHGQTELSEQ 181 RRYSGRGNPG LNEVGWRQVG AAAGYLARRG GIAAVVSSPL QRAYDTAVTA ARALALDVVV 241 DDDLVETDFG AWEGLTFAEA AERDPELHRR WLQDTSITPP GGESFDDVLR RVRRGRDRII 301 VGYEGATVLV VSHVTPIKML LRLALDAGSG VLYRLHLDLA SLSIAEFYAD GASSVRLVNQ 361 TGYL //