LOCUS SIU00833.1 324 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 adenosine kinase protein. ACCESSION LT708304-2247 PROTEIN_ID SIU00833.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="adok" /locus_tag="BQ2027_MB2225C" /note="Mb2225c, cbhK, len: 324 aa. Equivalent to Rv2202c, len: 324 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 324 aa overlap). Probable cbhK, carbohydrate kinase (but not ribose) (EC 2.7.-.-), similar to several e.g. AE000915_1 Methanobacterium thermoautotrop (309 aa) FASTA score: opt: 370, E(): 3.3e-18; 31.2% identity in 276 aa overlap. Low similarity to carbohydrate kinases, e.g. SW:RBSK_BACSU P36945 ribokinase (23.9% identity in 272 aa overlap); contains PS00583 pfkB family of carbohydrate kinases signature 1 Protein product from Mb2225c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2225c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P83736" /db_xref="InterPro:IPR002173" /db_xref="InterPro:IPR011611" /db_xref="InterPro:IPR029056" /db_xref="PDB:4UBE" /db_xref="UniProtKB/Swiss-Prot:P83736" BEGIN 1 MTIAVTGSIA TDHLMRFPGR FSEQLLPEHL HKVSLSFLVD DLVMHRGGVA GNMAFAIGVL 61 GGEVALVGAA GADFADYRDW LKARGVNCDH VLISETAHTA RFTCTTDVDM AQIASFYPGA 121 MSEARNIKLA DVVSAIGKPE LVIIGANDPE AMFLHTEECR KLGLAFAADP SQQLARLSGE 181 EIRRLVNGAA YLFTNDYEWD LLLSKTGWSE ADVMAQIDLR VTTLGPKGVD LVEPDGTTIH 241 VGVVPETSQT DPTGVGDAFR AGFLTGRSAG LGLERSAQLG SLVAVLVLES TGTQEWQWDY 301 EAAASRLAGA YGEHAAAEIV AVLA //