LOCUS SIU00823.1 370 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable anthranilate phosphoribosyltransferase TrpD protein. ACCESSION LT708304-2237 PROTEIN_ID SIU00823.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="trpD" /locus_tag="BQ2027_MB2215C" /note="Mb2215c, trpD, len: 370 aa. Equivalent to Rv2192c, len: 370 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 370 aa overlap). Probable trpD, anthranilate phosphoribosyltransferase (EC 2.4.2.18) (see citation below), similar to e.g. TRPD_LACCA|P17170, (43.2% identity in 308 aa overlap). Initiation codon uncertain, gtg at 4086 in MTCY190 favoured by homology but this has no clear ribosome binding site. Protein product from Mb2215c detected using SWATH mass spectrometry. Mb2215c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P66993" /db_xref="InterPro:IPR000312" /db_xref="InterPro:IPR005940" /db_xref="InterPro:IPR017459" /db_xref="InterPro:IPR035902" /db_xref="InterPro:IPR036320" /db_xref="UniProtKB/Swiss-Prot:P66993" BEGIN 1 MALSAEGSSG GSRGGSPKAE AASVPSWPQI LGRLTDNRDL ARGQAAWAMD QIMTGNARPA 61 QIAAFAVAMT MKAPTADEVG ELAGVMLSHA HPLPADTVPD DAVDVVGTGG DGVNTVNLST 121 MAAIVVAAAG VPVVKHGNRA ASSLSGGADT LEALGVRIDL GPDLVARSLA EVGIGFCFAP 181 RFHPSYRHAA AVRREIGVPT VFNLLGPLTN PARPRAGLIG CAFADLAEVM AGVFAARRSS 241 VLVVHGDDGL DELTTTTTST IWRVAAGSVD KLTFDPAGFG FARAQLDQLA GGDAQANAAA 301 VRAVLGGARG PVRDAVVLNA AGAIVAHAGL SSRAEWLPAW EEGLRRASAA IDTGAAEQLL 361 ARWVRFGRQI //