LOCUS SIU00818.1 400 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable long-chain-fatty-acid-CoA ligase fadD15 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE) protein. ACCESSION LT708304-2232 PROTEIN_ID SIU00818.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD15" /locus_tag="BQ2027_MB2210" /note="Mb2210, fadD15, len: 508 aa. Equivalent to 3' end of Rv2187, len: 600 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 508 aa overlap). Probable fadD15, long-chain-fatty-acid-CoA ligase (EC 6.2.1.3), similar to several e.g. P44446|LCFH_HAEIN PUTATIVE LONG-CHAIN-FATTY-ACID--CoA LIGASE from Haemophilus influenzae (607 aa), FASTA scores: (607 aa) opt: 992, E(): 0, (31.5% identity in 578 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. BELONGS TO THE ATP-DEPENDENT AMP-BINDING ENZYME FAMILY. TBparse score is 0.902. REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a frameshift due to single base deletion (t-*) leads to a shorter product with a different NH2 part compared to its homolog in Mycobacterium tuberculosis strain H37Rv. Protein product from Mb2210 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2210 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TYX8" /db_xref="InterPro:IPR000873" /db_xref="InterPro:IPR020845" /db_xref="InterPro:IPR042099" /db_xref="UniProtKB/Swiss-Prot:Q7TYX8" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTQSNLVHEI KGARAYHPTL LRKGERLLVF LPLAHVLARA ISMAAFHSKV TVGFTSDIKN 61 LLPMLAVFKP TVVVSVPRVF EKVYNTAEQN AANAGKGRIF AIAAQTAVDW SEACDRGGPG 121 LLLRAKHAVF DRLVYRKLRA ALGGNCRAAV SGGAPLGARL GHFYRGAGLT IYEGYGLSET 181 SGGVAISQFN DLKIGTVGKP VPGNSLRIAD DGELLVRGGV VFSGYWRNEQ ATTEAFTDGW 241 FKTGDLGAVD EDGFLTITGR KKEIIVTAGG KNVAPAVLED QLRAHPLISQ AVVVGDAKPF 301 IGALITIDPE AFEGWKQRNS KTAGASVGDL ATDPDLIAEI DAAVKQANLA VSHAESIRKF 361 RILPVDFTED TGELTPTMKV KRKVVAEKFA SDIEAIYNKE //