LOCUS SIU00722.1 589 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 transmembrane serine/threonine-protein kinase j pknj (protein kinase j) (stpk j) protein. ACCESSION LT708304-2136 PROTEIN_ID SIU00722.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pknJ" /locus_tag="BQ2027_MB2115" /note="Mb2115, pknJ, len: 589 aa. Equivalent to Rv2088, len: 589 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 589 aa overlap). Probable pknJ, transmembrane serine/threonine-protein kinase (EC 2.7.1.-) (see citation below), similar to other serine/threonine-protein kinases e.g. PKWA_THECU|P49695 putative serine/threonine-protein kinase (742 aa), FASTA scores: opt: 457, E(): 2.7e-15, (26.0% identity in 578 aa overlap); etc. Contains PS00108 Serine/Threonine protein kinases active-site signature. Contains Hank's kinase subdomain. BELONGS TO THE SER/THR FAMILY OF PROTEIN KINASES. Experimental studies show evidence of auto-phosphorylation. Protein product from Mb2115 detected using SWATH mass spectrometry. Mb2115 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65733" /db_xref="InterPro:IPR000719" /db_xref="InterPro:IPR008271" /db_xref="InterPro:IPR011009" /db_xref="InterPro:IPR026954" /db_xref="InterPro:IPR038232" /db_xref="UniProtKB/Swiss-Prot:P65733" BEGIN 1 MAHELSAGSV FAGYRIERML GAGGMGTVYL ARNPDLPRSE ALKVLAAELS RDLDFRARFV 61 READVAAGLD HPNIVAVHQR GQFEGRLWIA MQFVDGGNAE DALRAATMTT ARAVYVIGEV 121 AKALDYAHQQ GVIHRDIKPA NFLLSRAAGG DERVLLSDFG IARALGDTGL TSTGSVLATL 181 AYAAPEVLAG QGFDGRADLY SLGCALFRLL TGEAPFAAGA GAAVAVVAGH LHQPPPTVSD 241 RVPGLSAAMD AVIATAMAKD PMRRFTSAGE FAHAAAAALY GGATDGWVPP SPAPHVISQG 301 AVPGSPWWQH PVGSVTALAT PPGHGWPPGL PPLPRRPRRY RRGVAAVAAV MVVAAAAVTA 361 VTMTSHQPRT ATPPSAAALS PTSSSTTPPQ PPIVTRSRLP GLLPPLDDVK NFVGIQNLVA 421 HEPMLQPQTP NGSINPAECW PAVGGGVPSA YDLGTVIGFY GLTIDEPPTG TAPNQVGQLI 481 VAFRDAATAQ RHLADLASIW RRCGGRTVTL FRSEWRRPVE LSTSVPEVVD GITTMVLTAQ 541 GPVLRVREDH AIAAKNNVLV DVDIMTPDTS RGQQAVIGIT NYILAKIPG //