LOCUS SIU00706.1 62 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable precorrin-6y methyltransferase CobLa [FIRST PART] protein. ACCESSION LT708304-2120 PROTEIN_ID SIU00706.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cobLa" /locus_tag="BQ2027_MB2099C" /note="Mb2099c, cobLa, len: 62 aa. Similar to 5' end of Rv2072c, len: 390 aa, from Mycobacterium tuberculosis strain H37Rv, (97.727% identity in 44 aa overlap). Probable cobL, methyl transferase (EC 2.1.1.132), similar to L21196|g347169|RERCOBLMK1 from Rhodocococcus sp. NI86/21 (447 aa), FASTA scores: opt: 892; E(): 0; (50.1% identity in 369 aa overlap), and to COBL_PSEDE|P21921 precorrin-6y methylase (413 aa), FASTA scores: opt: 830, E(): 0, (40.6% identity in 404 aa overlap). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a large deletion of 2029 bp (H37Rv2.2330073-2332101-*) (RD9) leads to the loss of the NH2 part of cobL, the entire Rv2073 and Rv2074, and the COOH part of Mb2100c. In addition, while cobL exists as a single gene in Mycobacterium tuberculosis strain H37Rv, in Mycobacterium bovis a frameshift due to a single base insertion (*-t) splits cobL into 2 parts, cobLa and cobLb. Mb2099c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y058" /db_xref="InterPro:IPR035996" /db_xref="UniProtKB/TrEMBL:A0A1R3Y058" BEGIN 1 MLPAVQGLSP DGADLHVVAS GDPLLHGIGS TLIRLFGHDN VTVFAARVRG DVGVRPDGLE 61 RV //