LOCUS SIU00701.1 307 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CLASS A BETA-LACTAMASE BLAC protein. ACCESSION LT708304-2115 PROTEIN_ID SIU00701.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="blaC" /locus_tag="BQ2027_MB2094C" /note="Mb2094c, blaC, len: 307 aa. Equivalent to Rv2068c, len: 307 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 307 aa overlap). blaC, class A beta-lactamase (EC 3.5.2.6) (see citation below), similar to e.g. BLAC_NOCLA Q06316 beta-lactamase precursor (302 aa), FASTA scores, opt: 860, E(): 0, (50.2% identity in 283 aa overlap); eyc. Contains PS00013 Prokaryotic lipid attachment site near N-terminus, and PS00146 Beta-lactamase class-A active site. BELONGS TO THE CLASS-C BETA-LACTAMASE FAMILY. Protein product from Mb2094c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2094c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5I7" /db_xref="InterPro:IPR000871" /db_xref="InterPro:IPR012338" /db_xref="InterPro:IPR023650" /db_xref="UniProtKB/Swiss-Prot:P0A5I7" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MRNRGFGRRE LLVAMAMLVS VTGCARHASG ARPASTTLPA GADLADRFAE LERRYDARLG 61 VYVPATGTTA AIEYRADERF AFCSTFKAPL VAAVLHQNPL THLDKLITYT SDDIRSISPV 121 AQQHVQTGMT IGQLCDAAIR YSDGTAANLL LADLGGPGGG TAAFTGYLRS LGDTVSRLDA 181 EEPELNRDPP GDERDTTTPH AIALVLQQLV LGNALPPDKR ALLTDWMARN TTGAKRIRAG 241 FPADWKVIDK TGTGDYGRAN DIAVVWSPTG VPYVVAVMSD RAGGGYDAEP REALLAEAAT 301 CVAGVLA //