LOCUS SIU00678.1 511 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 carboxylesterase lipt protein. ACCESSION LT708304-2091 PROTEIN_ID SIU00678.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="lipT" /locus_tag="BQ2027_MB2071C" /note="Mb2071c, lipT, len: 511 aa. Equivalent to Rv2045c, len: 511 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 511 aa overlap). Probable lipT, carboxylesterase similar to many e.g. O08472 (489 aa) and P37967|PNBA_ BACSU (489 aa). PARA-NITROBENZYL ESTERASE (EC 3.1.1.-). Contains PS00941 Carboxylesterases type-B signature 2. Contains PS00122 Carboxylesterases type-B serine active site. FASTA scores: sptr|O08472|O08472 INTRACELLULAR ESTERASE B (489 aa) opt: 849, E(): 0, (36.2% identity in 489 aa overlap) and sp|P37967|PNBA_BACSU PARA-NITROBENZYL ESTERASE (489 aa) opt: 838, E(): 0, (36.0% identity in 489 aa overlap). TBparse score is 0 .918 Protein product from Mb2071c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb2071c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y007" /db_xref="InterPro:IPR002018" /db_xref="InterPro:IPR002168" /db_xref="InterPro:IPR019819" /db_xref="InterPro:IPR019826" /db_xref="InterPro:IPR029058" /db_xref="UniProtKB/TrEMBL:A0A1R3Y007" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MALESATVGS MHERTVRART ATGIVEGFTR DGVHRWRSIP YARAPVGSLR FRAPQPAQPW 61 PGVRHCHTFA NCAPQQRRYT VMGIGRYQTR SEDCLTLNVV TPEEPATQPL PVMVFIHGGG 121 YILGSSATPI YDGAALARRG CVYVSVNYRL GALGCLDLSS LSTPQITLDS NVYLRDLVLA 181 LRWVHDNIAE FGGDPGNVTI FGESAGAHIT ATLLAVPAAK GLFARAISES PAAGMVRSRE 241 VAAEFAARFA NLIGARTQDA ANALMQASPA QLVEAQHHLI RQGMRKRLGA FPIGPVFGDD 301 YLPMDPVEAM RSGRVHAVPL IVGTNAEEGR LFTRFLGMLP TNEPMVEELL SGMKPADRER 361 ITAAYPNYPA PSACIQLGGD FAFSSAAWQI AEAHGANAPT YLYRYDYAPR TLRWSGFGAT 421 HATELFAVFD IYRTRFGALL TAAADRRAAL RVSNEVQRRW RCFSQIGVPG DDWPAYTQDD 481 RAVLVFDRRC RIEFDPHQHR RIAWDGFSLA N //