LOCUS SIU00663.1 606 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CONSERVED HYPOTHETICAL PROTEIN [FIRST PART] protein. ACCESSION LT708304-2076 PROTEIN_ID SIU00663.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB2056C" /note="Mb2056c, -, len: 606 aa. Equivalent to 5' end of Rv2030c, len: 681 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 587 aa overlap). Conserved hypothetical protein that corresponds to products of two adjacent ORF's described previously MSGTUBDWN_4 (390 aa) and MSGTUBDWN_1 (385 aa). Also similar to C-terminal two-thirds of Mycobacterium tuberculosis protein Rv2143 (MTCY270.25c; 352 aa) and to Rv0571c (443 aa) and Mycobacterium leprae protein U650s MLU15184_16 (258 aa). FASTA scores: M93129|MSGTUBDWN_4 (390 aa) opt: 2530 E(): 0; 97.7% identity in 385 aa overlap and M93129|MSGTUBDWN_1 (385 aa) opt: 1983 E(): 0; 99.0% identity in 309 aa overlap. Z95388| MTCY270_25 (352 aa) opt: 882 E(): 0; 61.1 % identity in 226 aa overlap. U15184|MLU15184_16 (258 aa) opt: 549 E(): 9.8e-29; 43.8% identity in 219 aa overlap. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv2030c exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base deletion (g-*) splits Rv2030c into 2 parts, Mb2055c and Mb2056c. Protein product from Mb2056c detected using shotgun mass spectrometry. Mb2056c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y005" /db_xref="InterPro:IPR000836" /db_xref="InterPro:IPR007815" /db_xref="InterPro:IPR014622" /db_xref="InterPro:IPR029057" /db_xref="UniProtKB/TrEMBL:A0A1R3Y005" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLMTAAADVT RRSPRRVFRD RREAGRVLAE LLAAYRDQPD VIVLGLARGG LPVAWEVAAA 61 LHAPLDAFVV RKLGAPGHDE FAVGALASGG RVVVNDDVVR GLRITPQQLR DIAEREGREL 121 LRRESAYRGE RPPTDITGKT VIVVDDGLAT GASMFAAVQA LRDAQPAQIV IAVPAAPEST 181 CREFAGLVDD VVCATMPTPF LAVGESFWDF RQVTDEEVRR LLATPTAGPS LRRPAASTAA 241 DVLRRVAIDA PGGVPTHEVL AELVGDARIV LIGESSHGTH EFYQARAAMT QWLIEEKGFG 301 AVAAEADWPD AYRVNRYVRG LGEDTNADEA LSGFERFPAW MWRNTVVRDF VEWLRTRNQR 361 YESGALRQAG FYGLDLYSLH RSIQEVISYL DKVDPRAAAR ARARYACFDH ACADDGQAYG 421 FAAAFGAGPS CEREAVEQLV DVQRNALAYA RQDGLLAEDE LFYAQQNAQT VRDAEVYYRA 481 MFSGRVTSWN LRDQHMAQTL GSLLTHLDRH LDAPPARIVV WAHNSHVGDA RATEVWADGQ 541 LTLGQIVRER YGDESRSIGF STYTGTVTAA SEWGGIAQRK AVRPALHAVS RSSSTRLQTV 601 SWCQRG //