LOCUS SIU00563.1 620 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ACYL-COA LIGASE FADD31 (ACYL-COA SYNTHETASE) (ACYL-COA SYNTHASE) protein. ACCESSION LT708304-1976 PROTEIN_ID SIU00563.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD31" /locus_tag="BQ2027_MB1960" /note="Mb1960, fadD31, len: 620 aa. Equivalent to Rv1925, len: 620 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 620 aa overlap). Probable fadD31, acyl-CoA synthetase (EC 6.2.1.-), highly similar to others from Mycobacterium leprae e.g. NP_301198.1|NC_002677 putative acyl-CoA synthetase (635 aa); NP_302537.1|NC_002677 probable acyl-CoA synthase (583 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD32 (637 aa); fadD21 (578 aa); fadD29 (619 aa); fadD26|FD26_MYCTU|Q10976 (626 aa), FASTA scores: opt: 945, E(): 0, (39.8% identity in 598 aa overlap); etc. Also similar to N-terminus of G1171128 SAFRAMYCIN MX1 SYNTHETASE B from Myxococcus xanthus (1770 aa), FASTA scores: opt: 845, E(): 0, (37.4% identity in 593 aa overlap); N-terminus of T34918 polyketide synthase from Streptomyces coelicolor (2297 aa); etc. Protein product from Mb1960 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1960 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XZQ8" /db_xref="InterPro:IPR000873" /db_xref="InterPro:IPR040097" /db_xref="InterPro:IPR042099" /db_xref="UniProtKB/TrEMBL:A0A1R3XZQ8" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNDGSRQELR VRSGLLQIED CLDADGGIAL PAGTTLISLI ERNIKYVGDL VAYRYLDHAR 61 SAAGCALEVT WTQFGMRLAA IGAHVQRFAG PGDRVAILAP QGIDYVCGFY AAIKAGTVAV 121 PLFAPELPGH AERLDTALRD SEPAVILTTA AAKNAVEGFL NNVPRLRKPT VLVIDQIPDR 181 EGELFVPVEL DIDAVSHLQY TSGSTRPPVG VEITHRAVGT NLVQMILSID LLNRNTHGVS 241 WLPLYHDMGL SMIGFPAVYG GHSTLMSPTA FVRRPLRWIQ ALSEGSRTGR VVTAAPNFAY 301 EWAAQRGLPA QGDDVDLSNV VLIIGSEPVS IDAVTTFNKA FAPYGLPRTA FKPSYGIAEA 361 TLLVATIDHA AEPTVVYLDP EQLGAGHATR VAPDAPNAVV HVSCGHVARS LWAVIVDPDT 421 GPEAGAELPD GEIGEVWLQG DNVARGYWGR PEETRMTFGA RLQSPLAEGS HADGSAIDDT 481 WLRTGDLGVY LDGELYITGR IADLLTIDGR NHYPQDIEAT AAEASPMVRR GYITAFTVPA 541 SDGDDRNQRL VIIAERAAGT SRSDPRPALD AIRAAVCNRH GLSVADLSFL PAGAIPRTTS 601 GKLARQACRA QYLSGRLGVH //