LOCUS SIU00555.1 334 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PPE FAMILY PROTEIN protein. ACCESSION LT708304-1968 PROTEIN_ID SIU00555.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="PPE35b" /locus_tag="BQ2027_MB1952C" /note="Mb1952c, PPE35b, len: 334 aa. Equivalent to 3' end of Rv1918c, len: 987 aa, from Mycobacterium tuberculosis strain H37Rv, (99.1% identity in 334 aa overlap). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins. Similar to MTCY28.16|Z95890 M. tuberculosis cosmid (1053 aa), FASTA scores: opt: 3404, E(): 0, (65.6% identity in 1058 aa overlap). Also similar to MTV004.05, MTY13E10.17, MTV014.03, MTCY3C7.23, MTCY6G11.05, MTCY48.17, MTV004.03, MTCY31.07, MTCY4C12.36, MTCY180.01, etc. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, PPE35 exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base insertion (*-t) splits PPE35 into 2 parts, PPE35a and PPE35b. Mb1952c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="UniProtKB/TrEMBL:A0A1R3Y0I4" BEGIN 1 MVVFIPNNIT ALQTNMPGVF PQIGGFANTP PAFINTGTIT VGGGQINGVG FSIGAINVTP 61 FTLPNVVIQP WSLGGISVDG FTLPEISTQE FTTPALTISP IGVGALSLPD IITQQFTTPE 121 LTIDPITLGG FTLPQLSIPA ITTPAFTIDP IALGGFTLPQ IMTPEITTPP FAIDPIGLSG 181 FTLPQVNIPE ITTPEFTIQP VGLAAFTTPA LTIARIHLPS TTMGGFAIPA GPGYFNSSAT 241 PSSGFFNAGI GGNSGFGNSG SGLSGWFNTS PVGLLAGSGY QNYGGLISGF SNLGSGISGF 301 ANTGTLPFAV TSLVSGLANI GNNLSGLFFQ STTP //