LOCUS SIU00516.1 438 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable cytochrome p450 140 CYP140 protein. ACCESSION LT708304-1929 PROTEIN_ID SIU00516.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cyp140" /locus_tag="BQ2027_MB1912C" /note="Mb1912c, cyp140, len: 438 aa. Equivalent to Rv1880c, len: 438 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 438 aa overlap). Probable cyp140, cytochrome p450 (EC 1.14.-.-). Similar to Q00441|CPXJ_SACER 6-deoxyerythronolide beta hydroxylase (404 aa), FASTA scores: opt: 775, E(): 0, (44.2% identity in 319 aa overlap); and other members of the cytochrome P450 family. Related to Mycobacterium tuberculosis proteins include: Rv0766c, Rv2266, Rv0778, etc. Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). BELONGS TO THE CYTOCHROME P450 FAMILY. Protein product from Mb1912c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1912c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P63722" /db_xref="InterPro:IPR001128" /db_xref="InterPro:IPR002397" /db_xref="InterPro:IPR017972" /db_xref="InterPro:IPR036396" /db_xref="UniProtKB/Swiss-Prot:P63722" BEGIN 1 MKDKLHWLAM HGVIRGIAAI GIRRGDLQAR LIADPAVATD PVPFYDEVRS HGALVRNRAN 61 YLTVDHRLAH DLLRSDDFRV VSFGENLPPP LRWLERRTRG DQLHPLREPS LLAVEPPDHT 121 RYRKTVSAVF TSRAVSALRD LVEQTAINLL DRFAEQPGIV DVVGRYCSQL PIVVISEILG 181 VPEHDRPRVL EFGELAAPSL DIGIPWRQYL RVQQGIRGFD CWLEGHLQQL RHAPGDDLMS 241 QLIQIAESGD NETQLDETEL RAIAGLVLVA GFETTVNLLG NGIRMLLDTP EHLATLRQHP 301 ELWPNTVEEI LRLDSPVQLT ARVACRDVEV AGVRIKRGEV VVIYLAAANR DPAVFPDPHR 361 FDIERPNAGR HLAFSTGRHF CLGAALARAE GEVGLRTFFD RFPDVRAAGA GSRRDTRVLR 421 GWSTLPVTLG PARSMVSP //