LOCUS SIU00500.1 286 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE protein. ACCESSION LT708304-1913 PROTEIN_ID SIU00500.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1896C" /note="Mb1896c, -, len: 286 aa. Equivalent to Rv1865c, len: 286 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 286 aa overlap). Probable short-chain dehydrogenase (EC 1.-.-.-), highly similar to C-terminus of NP_301650.1|NC_00267 putative oxidoreductase from Mycobacterium leprae (596 aa). Also similar to various dehydrogenases, generally belonging to short-chain family, e.g. AAG02168.1|AF212041_24|AF212041 3-oxoacyl-(acylcarrier protein) reductase from Zymomonas mobilis (251 aa); P50198|LINX_PSEPA 2,5-DICHLORO-2,5-CYCLOHEXADIENE-1,4-DIOL DEHYDROGENASE from Sphingomonas paucimobilis (250 aa); NP_105680.1|NC_002678 sorbitol dehydrogenase (also similar to acetoin reductase) from Mesorhizobium loti (256 aa); etc. And highly similar to C-terminus of ephD|Rv2214c|MTCY190.25c from Mycobacterium tuberculosis (592 aa); and many other oxidoreductases from Mycobacterium tuberculosis e.g. Y00P_MYCTU|Q10402 putative oxidoreductase (650 aa), FASTA scores: opt: 439, E(): 8.9e-20, (32.5% identity in 280 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. BELONGS TO THE SHORT-CHAIN DEHYDROGENASES/REDUCTASES (SDR) FAMILY. Protein product from Mb1896c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1896c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XZJ8" /db_xref="InterPro:IPR002347" /db_xref="InterPro:IPR020904" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3XZJ8" BEGIN 1 MPGRTSIGVK IRDKVQDKVI AITGGARGIG LATAAALHNL GAKVAIGDID EAMAKESGAD 61 LDLDMYGKLD VTDPDSFSGF LDAVERQLGP IDVLVNNAGI MPVGRIVDEP DPVTRRILDI 121 NVYGVILGSK LAAQRMVPRG RGHVINVASL AGEIYAVGVA TYCASKHAVV AFTDSARLEY 181 RSAGVKFSMV LPSFVNTELI AGTGGIKGFK NAEPADIADA IVGLIVHPKP RVRVTKAAGS 241 MIVAQRFMPR QVSEGLNRLL GGEHVFTDDV DMEKRRTYEA RARGEE //