LOCUS       SIU00495.1               325 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 ALANINE
            AND PROLINE RICH SECRETED PROTEIN APA (FIBRONECTIN ATTACHMENT
            PROTEIN) (Immunogenic protein MPT32) (Antigen MPT-32)
            (45-kDa glycoprotein) (45/47 kDa antigen) protein.
ACCESSION   LT708304-1908
PROTEIN_ID  SIU00495.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="apa"
                     /locus_tag="BQ2027_MB1891"
                     /standard_name="mpt32; modD"
                     /note="Mb1891, apa, len: 325 aa. Equivalent to Rv1860,
                     len: 325 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.7% identity in 325 aa overlap). apa (alternate gene
                     names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted
                     protein, very similar to P46842|N43L_MYCLE from
                     Mycobacterium leprae (287 aa), FASTA scores: opt: 1166,
                     E(): 0, (66.4% identity in 298 aa overlap). Known to be
                     glycosylated fibronectin-binding protein (see some
                     citations). TBparse score is 0.924. Protein product from
                     Mb1891 detected using shotgun mass spectrometry and SWATH
                     mass spectrometry. Mb1891 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:O30620"
                     /db_xref="InterPro:IPR010801"
                     /db_xref="UniProtKB/Swiss-Prot:O30620"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MHQVDPNLTR RKGRLAALAI AAMASASLVT VAVPATANAD PEPAPPVPTT AASPPSTAAA
       61 PPAPATPVAP PPPAAANTPN AQPGDPNAAP PPADPNAPPP PVIAPNAPQP VRIDNPVGGF
      121 SFALPAGWVE SDAAHLDYGS ALLSKTTGDP PFPGQPPPVA NDTRIVLGRL DQKLYASAEA
      181 TDSKAAARLG SDMGEFYMPY PGTRINQETV SLDANGVSGS ASYYEVKFSD PSKPNGQIWT
      241 GVIGSPAANA PDAGPPQRWF VVWLGTANNP VDKGAAKALA ESIRPLVAPP PAPAPAPAEP
      301 APAPAPAGEV APTPTTPTPQ RTLPA
//