LOCUS SIU00456.1 808 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 possible preprotein translocase atpase seca2 protein. ACCESSION LT708304-1869 PROTEIN_ID SIU00456.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="secA2" /locus_tag="BQ2027_MB1852" /note="Mb1852, secA2, len: 808 aa. Equivalent to Rv1821, len: 808 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 808 aa overlap). Possible secA2, preprotein translocase, component of secretion apparatus, similar to several preprotein translocases e.g. P28366|SECA_BACSU preprotein translocase secA subunit from Bacillus subtilis (841 aa), FASTA scores: opt: 1424, E(): 0, (35.9% identity in 786 aa overlap). Equivalent to AL008609|MLCB1788.45 Preprotein translocase SecA 2 from Mycobacterium leprae (778 aa) (87.1% identity in 780 aa overlap). Also similar to Rv3240c|MTCY20B11.15c secA preprotein translocase from Mycobacterium tuberculosis (949 aa). COULD BE PART OF THE PROKARYOTIC PROTEIN TRANSLOCATION APPARATUS WHICH COMPRISE SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF|Rv2586c, SECG|Rv1440 AND SECY|Rv0732. Protein product from Mb1852 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1852 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P66786" /db_xref="InterPro:IPR000185" /db_xref="InterPro:IPR011115" /db_xref="InterPro:IPR011116" /db_xref="InterPro:IPR011130" /db_xref="InterPro:IPR014018" /db_xref="InterPro:IPR020937" /db_xref="InterPro:IPR026389" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR036266" /db_xref="InterPro:IPR036670" /db_xref="UniProtKB/Swiss-Prot:P66786" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNVHGCPRIA ACRCTDTHPR GRPAFAYRWF VPKTTRAQPG RLSSRFWRLL GASTEKNRSR 61 SLADVTASAE YDKEAADLSD EKLRKAAGLL NLDDLAESAD IPQFLAIARE AAERRTGLRP 121 FDVQLLGALR MLAGDVIEMA TGEGKTLAGA IAAAGYALAG RHVHVVTIND YLARRDAEWM 181 GPLLDAMGLT VGWITADSTP DERRTAYDRD VTYASVNEIG FDVLRDQLVT DVNDLVSPNP 241 DVALIDEADS VLVDEALVPL VLAGTTHRET PRLEIIRLVA ELVGDKDADE YFATDSDNRN 301 VHLTEHGARK VEKALGGIDL YSEEHVGTTL TEVNVALHAH VLLQRDVHYI VRDDAVHLIN 361 ASRGRIAQLQ RWPDGLQAAV EAKEGIETTE TGEVLDTITV QALINRYATV CGMTGTALAA 421 GEQLRQFYQL GVSPIPPNKP NIREDEADRV YITTAAKNDG IVEHITEVHQ RGQPVLVGTR 481 DVAESEELHE RLVRRGVPAV VLNAKNDAEE ARVIAEAGKY GAVTVSTQMA GRGTDIRLGG 541 SDEADHDRVA ELGGLHVVGT GRHHTERLDN QLRGRAGRQG DPGSSVFFSS WEDDVVAANL 601 DHNKLPMATD ENGRIVSPRT GSLLDHAQRV AEGRLLDVHA NTWRYNQLIA QQRAIIVERR 661 NTLLRTVTAR EELAELAPKR YEELSDKVSE ERLETICRQI MLYHLDRGWA DHLAYLADIR 721 ESIHLRALGR QNPLDEFHRM AVDAFASLAA DAIEAAQQTF ETANVLDHEP GLDLSKLARP 781 TSTWTYMVND NPLSDDTLSA LSLPGVFR //