LOCUS SIU00455.1 547 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable Acetolactate synthase ilvG (Acetohydroxy-acid synthase) (ALS) protein. ACCESSION LT708304-1868 PROTEIN_ID SIU00455.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ilvG" /locus_tag="BQ2027_MB1851" /note="Mb1851, ilvG, len: 547 aa. Equivalent to Rv1820, len: 547 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 547 aa overlap). Probable ilvG, acetolactate synthase (EC 4.1.3.18). Equivalent to AL008609|MLCB1788.46c ilvG from Mycobacterium leprae (548 aa) (86.1% identity in 548 aa overlap). Similar to ILVB_KLEPN|P27696 (559 aa), FASTA scores: opt: 660, E(): 2.9e-34, (29.1% identity in 549 aa overlap). Also similar to other Mycobacterium tuberculosis Ilv proteins e.g. Rv3003c (ilvB), etc. Contains PS00187 Thiamine pyrophosphate enzymes signature. Protein product from Mb1851 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1851 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P66947" /db_xref="InterPro:IPR000399" /db_xref="InterPro:IPR011766" /db_xref="InterPro:IPR012000" /db_xref="InterPro:IPR012001" /db_xref="InterPro:IPR029035" /db_xref="InterPro:IPR029061" /db_xref="UniProtKB/Swiss-Prot:P66947" BEGIN 1 MSTDTAPAQT MHAGRLIARR LKASGIDTVF TLSGGHLFSI YDGCREEGIR LIDTRHEQTA 61 AFAAEGWSKV TRVPGVAALT AGPGITNGMS AMAAAQQNQS PLVVLGGRAP ALRWGMGSLQ 121 EIDHVPFVAP VARFAATAQS AENAGLLVDQ ALQAAVSAPS GVAFVDFPMD HAFSMSSDNG 181 RPGALTELPA GPTPAGDALD RAAGLLSTAQ RPVIMAGTNV WWGHAEAALL RLVEERHIPV 241 LMNGMARGVV PADHRLAFSR ARSKALGEAD VALIVGVPMD FRLGFGGVFG STTQLIVADR 301 VEPAREHPRP VAAGLYGDLT ATLSALAGSG GTDHQGWIEE LATAETMARD LEKAELVDDR 361 IPLHPMRVYA ELAALLERDA LVVIDAGDFG SYAGRMIDSY LPGCWLDSGP FGCLGSGPGY 421 ALAAKLARPQ RQVVLLQGDG AFGFSGMEWD TLVRHNVAVV SVIGNNGIWG LEKHPMEALY 481 GYSVVAELRP GTRYDEVVRA LGGHGELVSV PAELRPALER AFASGLPAVV NVLTDPSVAY 541 PRRSNLA //