LOCUS SIU00441.1 409 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ppe family protein ppe32 protein. ACCESSION LT708304-1854 PROTEIN_ID SIU00441.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="PPE32" /locus_tag="BQ2027_MB1837" /note="Mb1837, PPE32, len: 409 aa. Equivalent to Rv1808, len: 409 aa, from Mycobacterium tuberculosis strain H37Rv, (100.000% identity in 409 aa overlap). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to Rv1800|MTV049.22|AL022021 (655 aa), FASTA scores: opt: 1225, E(): 0, (55.1% identity in 423 aa overlap). Contains PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. TBparse score is 0.919. Protein product from Mb1837 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1837 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="InterPro:IPR000030" /db_xref="InterPro:IPR022171" /db_xref="InterPro:IPR038332" /db_xref="UniProtKB/TrEMBL:A0A1R3XZC6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MDFGALPPEI NSGRMYAGPG SGPLLAAAAA WDALAAELYS AAASYGSTIE GLTVAPWMGP 61 SSITMAAAVA PYVAWISVTA GQAEQAGAQA KIAAGVYETA FAATVPPPVI EANRALLMSL 121 VATNIFGQNT PAIAATEAHY AEMWAQDAAA MYGYAGSSAT ASQLAPFSEP PQTTNPSATA 181 AQSAVVAQAA GAAASSDITA QLSQLISLLP STLQSLATTA TATSASAGWD TVLQSITTIL 241 ANLTGPYSII GLGAIPGGWW LTFGQILGLA QNAPGVAALL GPKAAAGALS PLAPLRGGYI 301 ADITPLGGGA TGGIARAIYV GSLSVPQGWA EAAPVMRAVA SVLPGTGAAP ALAAEAPGAL 361 FGEMALSSLA GRALAGTAVR SGAGAARVAG GSVTEDVAST TTIIVIPAD //