LOCUS SIU00436.1 87 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PE- PGRS FAMILY PROTEIN [FIRST PART] protein. ACCESSION LT708304-1849 PROTEIN_ID SIU00436.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="PE_PGRS32a" /locus_tag="BQ2027_MB1832C" /note="Mb1832c, PE_PGRS32a, len: 87 aa. Equivalent to 5' end of Rv1803c, len: 639 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 87 aa overlap). Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins. Most similar to Rv1768|MTCY28.34|Z95890 (618 aa), FASTA scores: opt: 1827, E(): 0, (53.5% identity in 664 aa overlap). Contains two PS00583 pfkB family of carbohydrate kinases signatures 1. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, PE_PGRS32 exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base transition (c-t) splits PE_PGRS32 into 2 parts, PE_PGRS32a and PE_PGRS32b, with PE_PGRS32a being truncated. Mb1832c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR000084" /db_xref="UniProtKB/TrEMBL:A0A1R3XZQ3" BEGIN 1 MWTSQMIVAP AFVDAAAKDL ATIGSAISRA NAEALVPITA LLPAGADDVS AAIAALFATH 61 GQAYQELSAH AVAFHEQFVQ LMSAGAA //