LOCUS SIU00428.1 585 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable proline rich membrane-anchored mycosin mycp5 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-5) protein. ACCESSION LT708304-1841 PROTEIN_ID SIU00428.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mycp5" /locus_tag="BQ2027_MB1824" /note="Mb1824, -, len: 585 aa. Equivalent to Rv1796, len: 585 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 585 aa overlap). Conserved hypothetical Pro-rich protease. Member of family with four other Mycobacterium tuberculosis hypothetical proteases including Rv3886c|O05458|MTCY15F10.26|Z94121 (550 aa), FASTA scores: opt: 1173, E(): 0, (47.9% identity in 578 aa overlap); Rv0291, Rv3883c, and Rv3449. Genes all linked to those of ESAT-6 family. Has possible N-terminal signal peptide and hydrophobic anchor-like stretch at C-terminus. Contains two serine protease, subtilase family active site motifs: a aspartic acid active site motif (PS00136); and a histidine active site motif (PS00137). Protein product from Mb1824 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1824 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XZD8" /db_xref="InterPro:IPR000209" /db_xref="InterPro:IPR015500" /db_xref="InterPro:IPR023827" /db_xref="InterPro:IPR023834" /db_xref="InterPro:IPR036852" /db_xref="UniProtKB/TrEMBL:A0A1R3XZD8" BEGIN 1 MQRFGTGSSR SWCGRAGTAT IAAVLLASGA LTGLPPAYAI SPPTIDPGAL PPDGPPGPLA 61 PMKQNAYCTE VGVLPGTDFQ LQPKYMEMLN LNEAWQFGRG DGVKVAVIDT GVTPHPRLPR 121 LIPGGDYVMA GGDGLSDCDA HGTLVASMIA AVPANGAVPL PSVPRRPVTI PTTETPPPPQ 181 TVTLSPVPPQ TVTVIPAPPP EEGVPPGAPV PGPEPPPAPG PQPPAVDRGG GTVTVPSYSG 241 GRKIAPIDNP RNPHPSAPSP ALGPPPDAFS GIAPGVEIIS IRQSSQAFGL KDPYTGDEDP 301 QTAQKIDNVE TMARAIVHAA NMGASVINIS DVMCMSARNV IDQRALGAAV HYAAVDKDAV 361 IVAAAGDGSK KDCKQNPIFD PLQPDDPRAW NAVTTVVTPS WFHDYVLTVG AVDANGQPLS 421 KMSIAGPWVS ISAPGTDVVG LSPRDDGLIN AIDGPDNSLL VPAGTSFSAA IVSGVAALVR 481 AKFPELSAYQ IINRLIHTAR PPARGVDNQV GYGVVDPVAA LTWDVPKGPA EPPKQLSAPL 541 VVPQPPAPRD MVPIWVAAGG LAGALLIGGA VFGTATLMRR SRKQQ //