LOCUS SIU00382.1 532 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE FATTY-ACID-COA LIGASE FADD1 (FATTY-ACID-COA SYNTHETASE) (FATTY-ACID-COA SYNTHASE) protein. ACCESSION LT708304-1795 PROTEIN_ID SIU00382.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD1" /locus_tag="BQ2027_MB1779C" /note="Mb1779c, fadD1, len: 532 aa. Equivalent to Rv1750c, len: 532 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 532 aa overlap). Possible fadD1, fatty-acid-CoA synthetase (EC 6.2.1.-), similar in part to others e.g. O35488|VLCS_MOUSE VERY-LONG-CHAIN ACYL-COA SYNTHETASE from Mus musculus (620 aa); NP_113924.1|NM_031736 solute carrier family 27 (fatty acid transporter) member 2 from Rattus norvegicus (620 aa); NP_459076.1|NC_003197 crotonobetaine/carnitine-CoA ligase from Salmonella typhimurium (517 aa); CAIC_ECOLI|P31552 probable crotonobetaine/carnitine-coa ligase from Escherichia coli (522 aa), FASTA scores: opt: 448, E(): 1.9e-21, (25.1% identity in 502 aa overlap); etc. Also highly similar to fadD17|Rv3506|MTV023.13 PROBABLE FATTY-ACID-COA LIGASE from Mycobacterium tuberculosis (502 aa); and similar to others from Mycobacterium tuberculosis e.g. fadD6|MTCI364.18|Rv1206|O05307 PROBABLE FATTY-ACID-COA LIGASE (597 aa), FASTA score: (28.3% identity in 519 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. BELONGS TO THE ATP-DEPENDENT AMP-BINDING ENZYME FAMILY. Protein product from Mb1779c detected using SWATH mass spectrometry. Mb1779c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XZ72" /db_xref="InterPro:IPR000873" /db_xref="InterPro:IPR020845" /db_xref="InterPro:IPR025110" /db_xref="InterPro:IPR030310" /db_xref="InterPro:IPR042099" /db_xref="UniProtKB/TrEMBL:A0A1R3XZ72" BEGIN 1 MTDTIQSLLR QHVSDPTIAV KYGGLQWTWS QYLAESAARA AALITIADPQ RPTHIGSLLG 61 NTPEMLAQLA AAGLGGYVLC GLNTTRRGDA LAADVRRADC QIVVTDADHR ALLDGLDLAG 121 ARILDTSTPR WAELVAGDGA FVPYREVDTM DPFMMIFTSG TSGNPKAVPV SHLMATFAGR 181 SLTERFGLTE QDTCYVSMPL FHSNAVVAGW APAVVSGAAI APATFSATGF LDDVRRYHAT 241 YMNYVGKPLA YILATPERDD DADNPLRVAF GNEANDKDIE EFSRRFGVQV EDGFGSTENA 301 VIVIREPGTP PGSIGRGAHG VAVYNGETVT ECAVARFDAH GALTNADEAI GELVNTTGSG 361 FFTGYYNDPE ANAERMRHGM YWSGDLAYRD SEGWIYLAGR TADWMRVDGE NLTAAPIERI 421 LLRYKAINRV AVYAVPDEYV GDQVMAALVL RAGDTFDPDA FEAFLDAQPD LSTKARPRYI 481 RIAADLPSTA THKVLKRQLI DEGTAVGKAD TLWVREPRGS AYHHASGPAK AI //