LOCUS SIU00333.1 196 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable catechol-o-methyltransferase protein. ACCESSION LT708304-1746 PROTEIN_ID SIU00333.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1729C" /note="Mb1729c, -, len: 196 aa. Equivalent to Rv1703c, len: 196 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 196 aa overlap). Probable catechol-o-methyltransferase (EC 2.1.1.6), most similar to COMT_HUMAN|P21964 soluble form of mammalian catechol o-methyltransferase (271 aa), FASTA scores: opt: 405, E(): 7 .8e-29, (38.9% identity in 190 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical methyltransferases Rv0187, Rv1220c. Protein product from Mb1729c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1729c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y172" /db_xref="InterPro:IPR002935" /db_xref="InterPro:IPR029063" /db_xref="UniProtKB/TrEMBL:A0A1R3Y172" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLATIDKFAY EKSMLINVGD EKGTLLDAAV RRADPALALE LGTYLGYGAL RIARAAPEAR 61 VYSVELAEAN ASNARRIWAH AGVDDRVVCV VGTIGDGGRT LDALTEHGFA TGTLDFVFLD 121 HDKKAYLPDL QSILDRGWLH PGSIVVADNV RVPGAPKYRA YMRRQQGMSW NTIEHKTHLE 181 YQTLVPDLVL ESEYLG //