LOCUS SIU00325.1 307 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 inorganic polyphosphate/atp-nad kinase ppnk (poly(p)/atp nad kinase) protein. ACCESSION LT708304-1738 PROTEIN_ID SIU00325.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ppnK" /locus_tag="BQ2027_MB1721" /note="Mb1721, ppnK, len: 307 aa. Equivalent to Rv1695, len: 307 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 307 aa overlap). Probable ppnK, inorganic polyphosphate/ATP-NAD kinase (EC 2.7.1.23), equivalent to Q49897|MLC1351.13C|Z95117|PPNK_MYCLE INORGANIC POLYPHOSPHATE/ATP-NAD KINASE from Mycobacterium leprae (311 aa) (87.9% identity in 305 aa overlap). Also similar to many e.g. P37768|PPNK_ECOLI PROBABLE INORGANIC POLYPHOSPHATE/ATP-NAD KINASE (292 aa), FASTA scores: opt: 384, E(): 1.7e-23, (33.5% identity in 233 aa overlap); etc. BELONGS TO THE NAD KINASE FAMILY. Protein product from Mb1721 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1721 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A5S7" /db_xref="InterPro:IPR002504" /db_xref="InterPro:IPR016064" /db_xref="InterPro:IPR017437" /db_xref="InterPro:IPR017438" /db_xref="UniProtKB/Swiss-Prot:P0A5S7" BEGIN 1 MTAHRSVLLV VHTGRDEATE TARRVEKVLG DNKIALRVLS AEAVDRGSLH LAPDDMRAMG 61 VEIEVVDADQ HAADGCELVL VLGGDGTFLR AAELARNASI PVLGVNLGRI GFLAEAEAEA 121 IDAVLEHVVA QDYRVEDRLT LDVVVRQGGR IVNRGWALNE VSLEKGPRLG VLGVVVEIDG 181 RPVSAFGCDG VLVSTPTGST AYAFSAGGPV LWPDLEAILV VPNNAHALFG RPMVTSPEAT 241 IAIEIEADGH DALVFCDGRR EMLIPAGSRL EVTRCVTSVK WARLDSAPFT DRLVRKFRLP 301 VTGWRGK //