LOCUS SIU00324.1 268 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 2'- o-methyltransferase tlya protein. ACCESSION LT708304-1737 PROTEIN_ID SIU00324.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="tlyA" /locus_tag="BQ2027_MB1720" /note="Mb1720, tlyA, len: 268 aa. Equivalent to Rv1694, len: 268 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 268 aa overlap). tlyA, cytotoxin/haemolysin homologue (see citations below), almost identical to NP_301968.1|NC_002677 cytotoxin/haemolysin homologue TlyA from Mycobacterium leprae (269 aa). TlyA homologues were also identified by PCR in M. avium, M. bovis BCG, but appeared absent in M. smegmatis, M. vaccae, M. kansasii, M. chelonae and M. phlei (see first citation below). Also highly similar to CAB83047.1|AJ271681 putative haemolysin from Mycobacterium ulcerans (281 aa); and similar to HLYA_TREHY|Q06803 pore-forming haemolysin/cytotoxin virulence determinant from Treponema hyodysenteriae (240 aa), FASTA scores: opt: 514, E():3e-30, (37.3% identity in 236 aa overlap). Mb1720 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XZV6" /db_xref="InterPro:IPR002877" /db_xref="InterPro:IPR002942" /db_xref="InterPro:IPR004538" /db_xref="InterPro:IPR029063" /db_xref="InterPro:IPR036986" /db_xref="UniProtKB/TrEMBL:A0A1R3XZV6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MARRARVDAE LVRRGLARSR QQAAELIGAG KVRIDGLPAV KPATAVSDTT ALTVVTDSER 61 AWVSRGAHKL VGALEAFAIA VAGRRCLDAG ASTGGFTEVL LDRGAAHVVA ADVGYGQLAW 121 SLRNDPRVVV LERTNARGLT PEAIGGRVDL VVADLSFISL ATVLPALVGC ASRDADIVPL 181 VKPQFEVGKG QVGPGGVVHD PQLRARSVLA VARRAQELGW HSVGVKASPL PGPSGNVEYF 241 LWLRTQTDRA LSAKGLEDAV HRAISEGP //