LOCUS SIU00309.1 373 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE ACYL-COA DEHYDROGENASE FADE16 protein. ACCESSION LT708304-1722 PROTEIN_ID SIU00309.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadE16" /locus_tag="BQ2027_MB1706" /note="Mb1706, fadE16, len: 373 aa. Equivalent to Rv1679, len: 373 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 373 aa overlap). Possible fadE16, acyl-CoA dehydrogenase (EC 1.3.99.-), similar to acyl/butyryl-CoA dehydrogenases e.g. NP_244665.1|NC_002570 acyl-CoA dehydrogenase from Bacillus halodurans (380 aa); NP_000008.1|NM_000017 acyl-Coenzyme A dehydrogenase from Homo sapiens (412 aa); Z99113|BSUB0010_119 from Bacillus subtilis (380 aa), FASTA scores: opt: 439, E(): 3.4e-20, (29.6% identity in 287 aa overlap); etc. Weakly similar to many dehydrogenases and to P31571|CAIA_ECOLI probable carnitine operon oxidoreductase from Escherichia coli (380 aa), FASTA scores: opt: 109, E(): 0.0066, (28.6% identity in 98 aa overlap). Protein product from Mb1706 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1706 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XZ09" /db_xref="InterPro:IPR006091" /db_xref="InterPro:IPR009075" /db_xref="InterPro:IPR009100" /db_xref="InterPro:IPR013786" /db_xref="InterPro:IPR036250" /db_xref="InterPro:IPR037069" /db_xref="UniProtKB/TrEMBL:A0A1R3XZ09" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MATPGVVQEV VSVAAEHAER VDTDCAFPAE AVDALRKTGL LGLVLPREIG GMGSGPVEFT 61 EVVAQLSAAC GSTAMIYLMH MAAAVTVAAS PPPGLPDLLA DMASGKQLGT LAFSEPGSRS 121 HFWAPVSTAS ADGDGIAVRA DKSWVTSAGF ADVYVVSVGS ADGAAGDVDL YAVPADTPGL 181 RVAGTFTGMG LRGNASAPMA VDIRIPDSYR LGEAGGGFGI MMQTVLPWFN LGNAAVSLGL 241 ATAATGAAVK HVGTARLEHL GGSLAELPTI RAQIARMGTT LAAQKAYLEV AANSVSSPDD 301 TTLTHVLGVK ASVNDAALTI TESAMRVCGG AAFSKHLPIE RAFRDARAGS VMAPTADALY 361 DFYGRAVTGL PLF //