LOCUS SIU00291.1 353 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 chalcone synthase pks10 protein. ACCESSION LT708304-1704 PROTEIN_ID SIU00291.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pks10" /locus_tag="BQ2027_MB1688" /note="Mb1688, pks10, len: 353 aa. Equivalent to Rv1660, len: 353 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 353 aa overlap). Possible pks10, chalcone synthase (EC 2.3.1.74), similar to BCSA_BACSU|P54157 putative chalcone synthase from B. subtilis (365 aa), FASTA scores: opt: 701, E(): 0, (33.1% identity in 362 aa overlap). Also similar to M. tuberculosis Rv1665|pks11 polyketide synthase (chalcone synthase); and Rv1372|pks18 polyketide synthase. Other upstream initiation sites are possible but homology suggests this start. Protein product from Mb1688 detected using SWATH mass spectrometry. Mb1688 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7VEV2" /db_xref="InterPro:IPR001099" /db_xref="InterPro:IPR011141" /db_xref="InterPro:IPR012328" /db_xref="InterPro:IPR016039" /db_xref="UniProtKB/Swiss-Prot:Q7VEV2" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSVIAGVFGA LPPYRYSQRE LTDSFVSIPD FEGYEDIVRQ LHASAKVNSR HLVLPLEKYP 61 KLTDFGEANK IFIEKAVDLG VQALAGALDE SGLRPEDLDV LITATVTGLA VPSLDARIAG 121 RLGLRADVRR VPLFGLGCVA GAAGVARLHD YLRGAPDGVA ALVSVELCSL TYPGYKPTLP 181 GLVGSALFAD GAAAVVAAGV KRAQDIGADG PDILDSRSHL YPDSLRTMGY DVGSAGFELV 241 LSRDLAAVVE QYLGNDVTTF LASHGLSTTD VGAWVTHPGG PKIINAITET LDLSPQALEL 301 TWRSLGEIGN LSSASVLHVL RDTIAKPPPS GSPGLMIAMG PGFCSELVLL RWH //