LOCUS SIU00247.1 472 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable pyruvate kinase pykA protein. ACCESSION LT708304-1660 PROTEIN_ID SIU00247.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pykA" /locus_tag="BQ2027_MB1643" /note="Mb1643, pykA, len: 472 aa. Equivalent to Rv1617, len: 472 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 472 aa overlap). Probable pykA, pyruvate kinase (EC 2.7.1.40). FASTA best: Q46078 PYRUVATE KINASE from CORYNEBACTERIUM GLUTAMICUM (475 aa), opt: 2221, E(): 0, (72.2% identity in 468 aa overlap). BELONGS TO THE PYRUVATE KINASE FAMILY. Protein product from Mb1643 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1643 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XYV1" /db_xref="InterPro:IPR001697" /db_xref="InterPro:IPR011037" /db_xref="InterPro:IPR015793" /db_xref="InterPro:IPR015795" /db_xref="InterPro:IPR015806" /db_xref="InterPro:IPR015813" /db_xref="InterPro:IPR036918" /db_xref="InterPro:IPR040442" /db_xref="UniProtKB/TrEMBL:A0A1R3XYV1" BEGIN 1 MTRRGKIVCT LGPATQRDDL VRALVEAGMD VARMNFSHGD YDDHKVAYER VRVASDATGR 61 AVGVLADLQG PKIRLGRFAS GATHWAEGET VRITVGACEG SHDRVSTTYK RLAQDAVAGD 121 RVLVDDGKVA LVVDAVEGDD VVCTVVEGGP VSDNKGISLP GMNVTAPALS EKDIEDLTFA 181 LNLGVDMVAL SFVRSPADVE LVHEVMDRIG RRVPVIAKLD KPEAIDNLEA IVLAFDAVMV 241 ARGDLGVELP LEEVPLVQKR AIQMARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV 301 LDGADALMLS GETSVGKYPL AAVRTMSRII CAVEENSTAA PPLTHIPRTK RGVISYAARD 361 IGERLDAKAL VAFTQSGDTV RRLARLHTPL PLLAFTAWPE VRSQLAMTWG TETFIVPKMQ 421 STDGMIRQVD KSLLELARYK RGDLVVIVAG APPGTVGSTN LIHVHRIGED DV //