LOCUS SIU00237.1 360 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable ionic transporter integral membrane protein chaA protein. ACCESSION LT708304-1650 PROTEIN_ID SIU00237.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="chaA" /locus_tag="BQ2027_MB1633" /note="Mb1633, chaA, len: 360 aa. Equivalent to Rv1607, len: 360 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 360 aa overlap). Probable chaA, ionic transporter integral membrane protein, putative calcium/proton antiporter, similar to many e.g. P31801|CHAA_ECOLI CALCIUM/PROTON ANTIPORTER from Escherichia coli (366 aa), FASTA scores: opt: 736, E(): 0, (35.9% identity in 351 aa overlap). Equivalent to Mycobacterium leprae AL049913|MLCB1610_21 (77.7% identity in 364 aa overlap). SEEMS TO BELONG TO THE CaCA FAMILY. Protein product from Mb1633 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1633 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XYU2" /db_xref="InterPro:IPR004837" /db_xref="UniProtKB/TrEMBL:A0A1R3XYU2" BEGIN 1 MLKRVPWTVV LPSLAFVALV LTWGKQIGPV VGLLAAVLLA GAVLAAVNHA EVVAARVGEP 61 FGSLVLAVAV TTIEVALIVA LMVSGGDDAA TLARDTVFAA VMITTNGIAG LSLLLGSLRY 121 GVTLFNPHGS GAALATVTTL ATLSLVLPTF TTSQSGPELS PGQLIFAGAA SLGLYVLFLF 181 TQTVRHRDFF LPVAQKGAVE DDSHADPPST RAALLSLGLL LVALVAVVGL AKVESPVIEE 241 VVSAAGFPQS FVGVVIATLV LLPETLAAAR AARQGRLQTS LNLAYGSAMA SIGLTIPTIA 301 LASLWLSGPL QLGLGAIQLV LLVLTVVVSV LTVVPGRATR LQGEVHLVLL AAYLFLAVVP //