LOCUS SIU00235.1 267 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable cyclase hisF protein. ACCESSION LT708304-1648 PROTEIN_ID SIU00235.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="hisF" /locus_tag="BQ2027_MB1631" /note="Mb1631, hisF, len: 267 aa. Equivalent to Rv1605, len: 267 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 267 aa overlap). Probable hisF, cyclase involved in histidine biosynthetic pathway, similar to many e.g. AF0304|AF030405_1 Corynebacterium glutamicum cyclase (257 aa), FASTA scores: opt: 1201, E(): 0, (71.9% identity in 256 aa overlap). BELONGS TO THE HISA / HISF FAMILY. Protein product from Mb1631 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1631 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:Q7VEW8" /db_xref="InterPro:IPR004651" /db_xref="InterPro:IPR006062" /db_xref="InterPro:IPR011060" /db_xref="InterPro:IPR013785" /db_xref="UniProtKB/Swiss-Prot:Q7VEW8" BEGIN 1 MYADRDLPGA GGLAVRVIPC LDVDDGRVVK GVNFENLRDA GDPVELAAVY DAEGADELTF 61 LDVTASSSGR ATMLEVVRRT AEQVFIPLTV GGGVRTVADV DSLLRAGADK VAVNTAAIAC 121 LDLLADMARQ FGSQCIVLSV DARTVPVGSA PTPSGWEVTT HGGRRGTGMD AVQWAARGAD 181 LGVGEILLNS MDADGTKAGF DLALLRAVRA AVTVPVIASG GAGAVEHFAP AVAAGADAVL 241 AASVFHFREL TIGQVKAALA AEGITVR //