LOCUS SIU00193.1 250 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Maltooligosyltreh alose synthase TreYa [FIRST PART] protein. ACCESSION LT708304-1606 PROTEIN_ID SIU00193.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="treYa" /locus_tag="BQ2027_MB1590C" /standard_name="glgY" /note="Mb1590c, treYa, len: 250 aa. Equivalent to the 5' end of Rv1563c, len: 765 aa, from Mycobacterium tuberculosis strain H37Rv, (97.4% identity in 191 aa overlap). treY (previously called glgY), maltooligosyl trehalose synthase, confirmed biochemically (see citation below). Strong similarity to Q44315|63343 TREY MALTOOLIGOSYL TREHALOSE SYNTHASE from ARTHROBACTER SP (775 aa), fasta scores: opt: 1953, E(): 0; (46.0% identity in 789 aa overlap). Some similarity to alpha-amylases and to MTCY48.03 (30.2% identity in 215 aa overlap). May catalyse conversion of maltodextrins to maltooligosyl trehaloses. Also similar to Mycobacterium tuberculosis glgB (Rv1326c), treZ (Rv1562c). REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv1563c/treY exists as a single gene. In Mycobacterium bovis, a large deletion of 806 bp splits Rv1563c in two parts, treYa and treYb. Mb1590c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y0U5" /db_xref="InterPro:IPR006047" /db_xref="InterPro:IPR017853" /db_xref="UniProtKB/TrEMBL:A0A1R3Y0U5" BEGIN 1 MAFPVISTYR VQMRGRSNGF GFTFADAENL LDYLDDLGVS HLYLSPILTA VGGSTHGYDV 61 TDPTTVSPEL GGSDGLARLS AAARSRGMGL IVDIVPSHVG VGKPEQNAWW WDVLKFGRSS 121 AYAEFFDIDW ELGDGRIILP LLGSDSDVAN LRVDGDLLRL GDLALPVAPG SGDGTGPAVH 181 DRQHSVWCGR RGVSSPGRHP CSVVATVHDD TVHPRHQTRR GRACPHRRAV PSAVAVGQVH 241 RPRPSHCARP //