LOCUS SIU00191.1 580 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Maltooligosyltreh alose trehalohydrolase TreZ protein. ACCESSION LT708304-1604 PROTEIN_ID SIU00191.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="treZ" /locus_tag="BQ2027_MB1588C" /standard_name="glgZ" /note="Mb1588c, treZ, len: 580 aa. Equivalent to Rv1562c, len: 580 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 580 aa overlap). treZ (previously called glgZ), Maltooligosyltrehalose trehalohydrolase, confirmed biochemically (see citation below). Similar to Q44316|D63343 TREZ MALTOOLIGOSYL TREHALOSE TREHALOHYDROLASE from ARTHROBACTER SP (598 aa), FASTA scores: opt: 2071, E(): 0, (52.2% identity in 582 aa overlap); also similar to 1,4-alpha-glucan branching enzymes e.g. GLGB_BACST|P30538 (639 aa), FASTA scores: opt: 313, E(): 3.8e-13, (27.5% identity in 462 aa overlap). Also similar to Mycobacterium tuberculosis proteins Rv1326c|glgB, and Rv1563c treY (previously glgY). Protein product from Mb1588c detected using SWATH mass spectrometry. Mb1588c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XYM9" /db_xref="InterPro:IPR006047" /db_xref="InterPro:IPR012768" /db_xref="InterPro:IPR013783" /db_xref="InterPro:IPR014756" /db_xref="InterPro:IPR017853" /db_xref="InterPro:IPR022567" /db_xref="UniProtKB/TrEMBL:A0A1R3XYM9" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPEFRVWAPK PALVRLDVNG AVHAMTRSAD GWWHTTVAAP ADARYGYLLD DDPTVLPDPR 61 SARQPDGVHA RSQRWEPPGQ FGAARTDTGW PGRSVEGAVI YELHIGTFTT AGTFDAAIEK 121 LDYLVDLGID FVELMPVNSF AGTRGWGYDG VLWYSVHEPY GGPDGLVRFI DACHTRRLGV 181 LIDAVFNHLG PSGNYLPRFG PYLSSASNPW GDGINIAGAD SDEVRHYIID CALRWMRDFH 241 ADGLRLDAVH ALVDTTAVHV LEELANATRW LSGQLGRPLS LIAETDRNDP RLITRPSHGG 301 YGITAQWNDD IHHAIHTAVS GERQGYYADF GSLATLAYTL RNGYFHAGTY SSFRRRRHGR 361 ALDTSAIPAT RLLAYTCTHD QVGNRALGDR PSQYLTGGQL AIKAALTLGS PYTAMLFMGE 421 EWGASSPFQF FCSHPEPELA HSTVAGRKEE FAEHGWAADD IPDPQDPQTF QRCKLNWAEA 481 GSGEHARLHR FYRDLIALRH NEADLADPWL DHLMVDYDEQ QRWVVMRRGQ LMIACNLGAE 541 PTCVPVSGEL VLAWESPIIG DNSTELAAYS LAILRAAEPA //