LOCUS       SIU00179.1               647 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            fatty-acid-coa ligase fadd11 (fatty-acid-coa synthetase)
            (fatty-acid-coa synthase) protein.
ACCESSION   LT708304-1592
PROTEIN_ID  SIU00179.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadD11"
                     /locus_tag="BQ2027_MB1576"
                     /note="Mb1576, fadD11, len: 647 aa. Equivalent to Rv1549
                     (fadD11') and Rv1550 (fadD11), len: 175 aa and 571 aa,
                     from Mycobacterium tuberculosis strain H37Rv, (100%
                     identity in 167 aa overlap and 99.8% identity in 470 aa
                     overlap). Possible fadD11', fatty-acid-CoA synthetase (EC
                     6.2.1.-), similar to the N-terminus of many fatty-acid CoA
                     synthetases e.g. NP_147860.1|NC_000854
                     long-chain-fatty-acid--CoA ligase from Aeropyrum pernix
                     (651 aa); P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 (EC
                     6.2.1.12) from Solanum tuberosum (Potato) (545 aa), FASTA
                     scores: opt: 168, E(): 4.4e-06, (30.4% identity in 112 aa
                     overlap); etc. Possible frameshift with respect to next
                     ORF Rv1550|MTCY48.15c but we can find no sequence error to
                     account for this. Probable fadD11, fatty-acid-CoA
                     synthetase (EC 6.2.1.-), similar, except in N-terminus, to
                     many e.g. SC6A5.39|T35430 probable
                     long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) from
                     Streptomyces coelicolor (612 aa); NP_301672.1|NC_002677
                     putative long-chain-fatty-acid-CoA ligase from
                     Mycobacterium leprae (600 aa); P44446|LCFH_HAEIN putative
                     long-chain-fatty-acid-CoA ligase from Haemophilus
                     influenzae (607 aa), FASTA scores: opt: 762, E(): 2.3e-38,
                     (34.4% identity in 436 aa overlap); etc. Contains PS00455
                     Putative AMP-binding domain signature. BELONGS TO THE
                     ATP-DEPENDENT AMP-BINDING ENZYME FAMILY.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, Rv1549 and Rv1550 exist as 2
                     genes. In Mycobacterium bovis, a two single base
                     insertions (*-c and *-c) lead to a single product. Protein
                     product from Mb1576 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb1576 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XYM2"
                     /db_xref="InterPro:IPR000873"
                     /db_xref="InterPro:IPR020845"
                     /db_xref="InterPro:IPR042099"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XYM2"
BEGIN
        1 MGRHGSRWSR PPCFRVLRLW TYAHRCDLGH TDPLSRRTEM TTTERPTTMC EAFQRTAVMD
       61 PDAVALRTPG GNQTMTWRDY AAQVRRVAAG LAGLGVRRGD TVSLMMANRI EFYPLDVGAQ
      121 HVGATSFSVY NTLPAEQLTY VFDNAGTKVV ICEQQYVDRV RASGVPIEHI VCVDGAPPGT
      181 LSLTDLYAAA SGDFFDFEST WRAVQPEDIV TLIYTSGTTG NPKGVEMTHA NLLFEGYAID
      241 EVLGIRFGDR VTSFLPSAHI ADRMTGLYLQ EMFGTQVTAV ADARTIAAAL PDVRPTVWGA
      301 VPRVWEKLKA GIEFTVARET DEMKRQALAW AMSVAGKRAN ALLAGESMSD QLVAEWAKAD
      361 ESVLSKLRER LGFGELRWAL SGAAPIPKET LAFFAGIGIP IAEIWGMSEL SCVATASHPR
      421 DGRLGTVGKL LPGLQGKIAE DGEYLVRGPL VMKGYRKEPA KTAEAIDSDG WLHTGDVFDI
      481 DSDGYLRVVD RKKELIINAA GKNMSPANIE NTILAACPMV GVMMAIGDGR TYNTALLVFD
      541 ADSLGPYAAQ RGLDASPAAL AADPEVIARI AAGVAEGNAK LSRVEQIKRF RILPTLWEPG
      601 GDEITLTMKL KRRRIAAKYS AEIEELYASE LRPQVYEPAA VPSTQPA
//