LOCUS SIU00179.1 647 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable fatty-acid-coa ligase fadd11 (fatty-acid-coa synthetase) (fatty-acid-coa synthase) protein. ACCESSION LT708304-1592 PROTEIN_ID SIU00179.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD11" /locus_tag="BQ2027_MB1576" /note="Mb1576, fadD11, len: 647 aa. Equivalent to Rv1549 (fadD11') and Rv1550 (fadD11), len: 175 aa and 571 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 167 aa overlap and 99.8% identity in 470 aa overlap). Possible fadD11', fatty-acid-CoA synthetase (EC 6.2.1.-), similar to the N-terminus of many fatty-acid CoA synthetases e.g. NP_147860.1|NC_000854 long-chain-fatty-acid--CoA ligase from Aeropyrum pernix (651 aa); P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 (EC 6.2.1.12) from Solanum tuberosum (Potato) (545 aa), FASTA scores: opt: 168, E(): 4.4e-06, (30.4% identity in 112 aa overlap); etc. Possible frameshift with respect to next ORF Rv1550|MTCY48.15c but we can find no sequence error to account for this. Probable fadD11, fatty-acid-CoA synthetase (EC 6.2.1.-), similar, except in N-terminus, to many e.g. SC6A5.39|T35430 probable long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) from Streptomyces coelicolor (612 aa); NP_301672.1|NC_002677 putative long-chain-fatty-acid-CoA ligase from Mycobacterium leprae (600 aa); P44446|LCFH_HAEIN putative long-chain-fatty-acid-CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: opt: 762, E(): 2.3e-38, (34.4% identity in 436 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. BELONGS TO THE ATP-DEPENDENT AMP-BINDING ENZYME FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv1549 and Rv1550 exist as 2 genes. In Mycobacterium bovis, a two single base insertions (*-c and *-c) lead to a single product. Protein product from Mb1576 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1576 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XYM2" /db_xref="InterPro:IPR000873" /db_xref="InterPro:IPR020845" /db_xref="InterPro:IPR042099" /db_xref="UniProtKB/TrEMBL:A0A1R3XYM2" BEGIN 1 MGRHGSRWSR PPCFRVLRLW TYAHRCDLGH TDPLSRRTEM TTTERPTTMC EAFQRTAVMD 61 PDAVALRTPG GNQTMTWRDY AAQVRRVAAG LAGLGVRRGD TVSLMMANRI EFYPLDVGAQ 121 HVGATSFSVY NTLPAEQLTY VFDNAGTKVV ICEQQYVDRV RASGVPIEHI VCVDGAPPGT 181 LSLTDLYAAA SGDFFDFEST WRAVQPEDIV TLIYTSGTTG NPKGVEMTHA NLLFEGYAID 241 EVLGIRFGDR VTSFLPSAHI ADRMTGLYLQ EMFGTQVTAV ADARTIAAAL PDVRPTVWGA 301 VPRVWEKLKA GIEFTVARET DEMKRQALAW AMSVAGKRAN ALLAGESMSD QLVAEWAKAD 361 ESVLSKLRER LGFGELRWAL SGAAPIPKET LAFFAGIGIP IAEIWGMSEL SCVATASHPR 421 DGRLGTVGKL LPGLQGKIAE DGEYLVRGPL VMKGYRKEPA KTAEAIDSDG WLHTGDVFDI 481 DSDGYLRVVD RKKELIINAA GKNMSPANIE NTILAACPMV GVMMAIGDGR TYNTALLVFD 541 ADSLGPYAAQ RGLDASPAAL AADPEVIARI AAGVAEGNAK LSRVEQIKRF RILPTLWEPG 601 GDEITLTMKL KRRRIAAKYS AEIEELYASE LRPQVYEPAA VPSTQPA //