LOCUS SIU00168.1 326 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Probable L-aparaginase ansA protein. ACCESSION LT708304-1581 PROTEIN_ID SIU00168.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ansA" /locus_tag="BQ2027_MB1565C" /note="Mb1565c, ansA, len: 326 aa. Equivalent to Rv1538c, len: 326 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 326 aa overlap). Probable ansA, L-aparaginase, most similar to ASPG_BACLI|P30363 L-asparaginase (322 aa), FASTA scores: opt: 417, E(): 8.8e-19, (30.9% identity in 314 aa overlap). Contains PS00917 Asparaginase / glutaminase active site signature 2. Protein product from Mb1565c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1565c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P63628" /db_xref="InterPro:IPR004550" /db_xref="InterPro:IPR006034" /db_xref="InterPro:IPR020827" /db_xref="InterPro:IPR027473" /db_xref="InterPro:IPR027474" /db_xref="InterPro:IPR027475" /db_xref="InterPro:IPR036152" /db_xref="InterPro:IPR037152" /db_xref="InterPro:IPR040919" /db_xref="UniProtKB/Swiss-Prot:P63628" BEGIN 1 MGANHVRNDP IMARLTVITT GGTISTTAGP DGVLRPTHCG ATLIAGLDMD SDIEVVDLMA 61 LDSSKLTPAD WDRIGAAVQE AFRGGADGVV ITHGTDTLEE TALWLDLTYA GSRPVVLTGA 121 MLSADAPGAD GPANLRDALA VAADPAARDL GVLVSFGGRV LQPLGLHKVA NPDLCGFAGE 181 SLGFTSGGVR LTRTKTRPYL GDLGAAVAPR VDIVAVYPGS DAVAMDACVA AGARAVVLEA 241 LGSGNAGAAV IEGVRRHCRD GSDPVVIAVS TRVAGARVGA GYGPGHDLVE AGAVMVPRLP 301 PSQARVLLMA ALAANSPVAD VIDRWG //