LOCUS SIU00137.1 429 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ESTERASE LIPL protein. ACCESSION LT708304-1550 PROTEIN_ID SIU00137.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="lipL" /locus_tag="BQ2027_MB1534" /note="Mb1534, lipL, len: 429 aa. Equivalent to Rv1497, len: 429 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 429 aa overlap). Probable LipL, esterase (EC 3.1.-.-), very similar to Mycobacterium tuberculosis hypothetical esterases and penicillin binding proteins e.g. Rv1923, Rv2463, Rv3775, etc. Also similar to G151214|M68491 esterase estA from Pseudomonas sp (389 aa), FASTA scores: opt: 604, E(): 1e-31, (34.4% identity in 389 aa overlap). Protein product from Mb1534 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1534 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR001466" /db_xref="InterPro:IPR012338" /db_xref="UniProtKB/TrEMBL:A0A1R3XYJ7" BEGIN 1 MMVDTGVDHR AVSSHDGPDA GRRVFGAADP RFACVVRAFA SMFPGRRFGG GALAVYLDGQ 61 PVVDVWKGWA DRAGWVPWSA DSAPMVFSAT KGMTATVIHR LADRGLIDYE APVAEYWPAF 121 GANGKATLTV RDVMRHQAGL SGLRGATQQD LLDHVVMEER LAAAVPGRLL GKSAYHALTF 181 GWLMSGLARA VTGKDMRLLF REELAEPLDT DGLHLGRPPA DAPTRVAEII MPQDIAANAV 241 LTCAMRRLAH RFSGGFRSMY FPGAIAAVQG EAPLLDAEIP AANGVATARA LARMYGAIAN 301 GGEIDGIRFL SRELVTGLTR NRRQVLPDRN LLVPLNFHLG YHGMPIGNVM PGFGHVGLGG 361 SIGWTDPETG VAFALVHNRL LSPLVMTDHA GFVGIYHLIR QAAAQARKRG YQPVTPFGAP 421 YSEPGAAAG //