LOCUS SIU00133.1 750 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE METHYLMALONYL-COA MUTASE LARGE SUBUNIT MUTB (MCM) protein. ACCESSION LT708304-1546 PROTEIN_ID SIU00133.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mutB" /locus_tag="BQ2027_MB1530" /note="Mb1530, mutB, len: 750 aa. Equivalent to Rv1493, len: 750 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 750 aa overlap). Probable mutB, Methylmalonyl-coa mutase large-subunit (EC 5.4.99.2), strong similarity to e.g. MUTB_STRCM|Q05065 methylmalonyl-coa mutase alpha-subunit from Streptomyces cinnamonensis (733 aa), FASTA scores: opt: 3562, E(): 0, (75.8% identity in 730 aa overlap). Contains PS00544 Methylmalonyl-CoA mutase signature. BELONGS TO THE METHYLMALONYL-COA MUTASE FAMILY. Protein product from Mb1530 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1530 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65488" /db_xref="InterPro:IPR006098" /db_xref="InterPro:IPR006099" /db_xref="InterPro:IPR006158" /db_xref="InterPro:IPR006159" /db_xref="InterPro:IPR016176" /db_xref="InterPro:IPR036724" /db_xref="UniProtKB/Swiss-Prot:P65488" BEGIN 1 MTTKTPVIGS FAGVPLHSER AAQSPTEAAV HTHVAAAAAA HGYTPEQLVW HTPEGIDVTP 61 VYIAADRAAA EAEGYPLHSF PGEPPFVRGP YPTMYVNQPW TIRQYAGFST AADSNAFYRR 121 NLAAGQKGLS VAFDLATHRG YDSDHPRVQG DVGMAGVAID SILDMRQLFD GIDLSTVSVS 181 MTMNGAVLPI LALYVVAAEE QGVAPEQLAG TIQNDILKEF MVRNTYIYPP KPSMRIISDI 241 FAYTSAKMPK FNSISISGYH IQEAGATADL ELAYTLADGV DYIRAGLNAG LDIDSFAPRL 301 SFFWGIGMNF FMEVAKLRAG RLLWSELVAQ FAPKSAKSLS LRTHSQTSGW SLTAQDVFNN 361 VARTCIEAMA ATQGHTQSLH TNALDEALAL PTDFSARIAR NTQLVLQQES GTTRPIDPWG 421 GSYYVEWLTH RLARRARAHI AEVAEHGGMA QAISDGIPKL RIEEAAARTQ ARIDSGQQPV 481 VGVNKYQVPE DHEIEVLKVE NSRVRAEQLA KLQRLRAGRD EPAVRAALAE LTRAAAEQGR 541 AGADGLGNNL LALAIDAARA QATVGEISEA LEKVYGRHRA EIRTISGVYR DEVGKAPNIA 601 AATELVEKFA EADGRRPRIL IAKMGQDGHD RGQKVIATAF ADIGFDVDVG SLFSTPEEVA 661 RQAADNDVHV IGVSSLAAGH LTLVPALRDA LAQVGRPDIM IVVGGVIPPG DFDELYAAGA 721 TAIFPPGTVI ADAAIDLLHR LAERLGYTLD //