LOCUS SIU00132.1 615 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE METHYLMALONYL-COA MUTASE SMALL SUBUNIT MUTA (MCM) protein. ACCESSION LT708304-1545 PROTEIN_ID SIU00132.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mutA" /locus_tag="BQ2027_MB1529" /note="Mb1529, mutA, len: 615 aa. Equivalent to Rv1492, len: 615 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 615 aa overlap). Probable mutA, Methylmalonyl-coa mutase small-subunit (EC 5.4.99.2), strong similarity to e.g. MUTA_STRCM|Q05064 methylmalonyl-coa mutase beta-subunit from Streptomyces cinnamonensis (616 aa), FASTA scores: opt: 1512, E(): 0, (45.9% identity in 628 aa overlap). Contains PS00213 Lipocalin signature, PS00544 Methylmalonyl-CoA mutase signature. BELONGS TO THE METHYLMALONYL-COA MUTASE FAMILY. Protein product from Mb1529 detected using shotgun mass spectrometry. Mb1529 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65486" /db_xref="InterPro:IPR004608" /db_xref="InterPro:IPR006099" /db_xref="InterPro:IPR016176" /db_xref="InterPro:IPR036724" /db_xref="UniProtKB/Swiss-Prot:P65486" BEGIN 1 MSIDVPERAD LEQVRGRWRN AVAGVLSKSN RTDSAQLGDH PERLLDTQTA DGFAIRALYT 61 AFDELPEPPL PGQWPFVRGG DPLRDVHSGW KVAEAFPANG ATADTNAAVL AALGEGVSAL 121 LIRVGESGVA PDRLTALLSG VYLNLAPVIL DAGADYRPAC DVMLALVAQL DPGQRDTLSI 181 DLGADPLTAS LRDRPAPPIE EVVAVASRAA GERGLRAITV DGPAFHNLGA TAATELAATV 241 AAAVAYLRVL TESGLVVSDA LRQISFRLAA DDDQFMTLAK MRALRQLWAR VAEVVGDPGG 301 GAAVVHAETS LPMMTQRDPW VNMLRCTLAA FGAGVGGADT VLVHPFDVAI PGGFPGTAAG 361 FARRIARNTQ LLLLEESHVG RVLDPAGGSW FVEELTDRLA RRAWQRFQAI EARGGFVEAH 421 DFLAGQIAEC AARRADDIAH RRLAITGVNE YPNLGEPALP PGDPTSPVRR YAAGFEALRD 481 RSDHHLARTG ARPRVLLLPL GPLAEHNIRT TFATNLLASG GIEAIDPGTV DAGTVGNAVA 541 DAGSPSVAVI CGTDARYRDE VADIVQAARA AGVSRVYLAG PEKALGDAAH RPDEFLTAKI 601 NVVQALSNLL TRLGA //