LOCUS SIU00099.1 846 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Iron- sulfur cluster assembly protein SufB protein. ACCESSION LT708304-1512 PROTEIN_ID SIU00099.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB1496" /note="Mb1496, -, len: 846 aa. Equivalent to Rv1461, len: 846 aa, from Mycobacterium tuberculosis strain H37Rv, (100% identity in 846 aa overlap). Conserved hypothetical protein. Equivalent of spliced protein from Mycobacterium leprae MLCL536.28c len: 869. Residues 1-253 represent N-extein, and 613-846 the C-extein. The intein present from residues 254 - 612 is different in sequence and site of the insertion from the one present in MLCL536.28c. FASTA scores: Z99125|MLCL536_23 Mycobacterium leprae cosmid L536 (869 aa), opt: 1498 E(): 0, (54.1% identity in 917 aa overlap). The mature protein is similar to Z99120|BSUB0017_150 hypothetical Bacillus subtilis protein (465 aa), FASTA scores: opt:1053, E(): 0, (34.8% identity in 821 aa overlap). The intein shows some similarity to inteins from U67548|MJU67548_6 Methanococcus jannaschii (895 aa), FASTA scores: opt: 181, E(): 0.00023, (25.2% identity in 274 aa overlap). Protein product from Mb1496 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb1496 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P67126" /db_xref="InterPro:IPR000825" /db_xref="InterPro:IPR003586" /db_xref="InterPro:IPR003587" /db_xref="InterPro:IPR004042" /db_xref="InterPro:IPR006141" /db_xref="InterPro:IPR006142" /db_xref="InterPro:IPR010231" /db_xref="InterPro:IPR027434" /db_xref="InterPro:IPR030934" /db_xref="InterPro:IPR036844" /db_xref="InterPro:IPR037284" /db_xref="UniProtKB/Swiss-Prot:P67126" BEGIN 1 MTLTPEASKS VAQPPTQAPL TQEEAIASLG RYGYGWADSD VAGANAQRGL SEAVVRDISA 61 KKNEPDWMLQ SRLKALRIFD RKPIPKWGSN LDGIDFDNIK YFVRSTEKQA ASWDDLPEDI 121 RNTYDRLGIP EAEKQRLVAG VAAQYESEVV YHQIREDLEA QGVIFLDTDT GLREHPDIFK 181 EYFGTVIPAG DNKFSALNTA VWSGGSFIYV PPGVHVDIPL QAYFRINTEN MGQFERTLII 241 ADEGSYVHYV EGCLPAGELI TTADGDLRPI ESIRVGDFVT GHDGRPHRVT AVQVRDLDGE 301 LFTFTPMSPA NAFSVTAEHP LLAIPRDEVR VMRKERNGWK AEVNSTKLRS AEPRWIAAKD 361 VAEGDFLIYP KPKPIPHRTV LPLEFARLAG YYLAEGHACL TNGCESLIFS FHSDEFEYVE 421 DVRQACKSLY EKSGSVLIEE HKHSARVTVY TKAGYAAMRD NVGIGSSNKK LSDLLMRQDE 481 TFLRELVDAY VNGDGNVTRR NGAVWKRVHT TSRLWAFQLQ SILARLGHYA TVELRRPGGP 541 GVIMGRNVVR KDIYQVQWTE GGRGPKQARD CGDYFAVPIK KRAVREAHEP VYNLDVENPD 601 SYLAYGFAVH NCTAPIYKSD SLHSAVVEII VKPHARVRYT TIQNWSNNVY NLVTKRARAE 661 AGATMEWIDG NIGSKVTMKY PAVWMTGEHA KGEVLSVAFA GEDQHQDTGA KMLHLAPNTS 721 SNIVSKSVAR GGGRTSYRGL VQVNKGAHGS RSSVKCDALL VDTVSRSDTY PYVDIREDDV 781 TMGHEATVSK VSENQLFYLM SRGLTEDEAM AMVVRGFVEP IAKELPMEYA LELNRLIELQ 841 MEGAVG //